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Journal of Neuroscience, Vol 1, 176-191, Copyright © 1981 by Society for Neuroscience
Autoradiographic localization of muscarinic cholinergic receptors in rat brainstem
JK Wamsley, MS Lewis, WS Young 3d and MJ Kuhar
We have localized muscarinic cholinergic receptors in the rat brainstem by
a light microscopic autoradiographic method. Initially, we examined the
kinetics and specificity of the binding of radiolabeled quinuclidinyl
benzilate ([3H]QNB) in slide-mounted, frozen tissue sections to determine
that we were labeling the muscarinic cholinergic receptors as they had been
described previously in biochemical pharmacological studies. Subsequently,
using optimal binding parameters to obtain high specific to nonspecific
ratios, we labeled tissue sections and generated autoradiograms by the
apposition of emulsion- coated coverslips. We found high concentrations of
autoradiographic grains associated with many nuclei in the brainstem
including the superior colliculus, nuclei of the lateral lemniscus, pontine
nucleus, parabrachial nucleus, trigeminal nerve nucleus (V), the tegmental
nuclei, nucleus of the facial nerve (VII), the locus coeruleus, the medial
vestibular nucleus, cochlear nucleus, of the tractus solitarius, and
nucleus of the hypoglossal nerve (XII). We conclude that muscarinic
cholinergic effects of acetylcholine and important in the normal function
of both the sensory and motor systems. We speculate on the possible
mechanisms of action of certain cholinergic agents, such as the mechanism
of anticholinergic compounds when used to treat motion sickness or
anticholinesterase poisoning.
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