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Journal of Neuroscience, Vol 1, 192-203, Copyright © 1981 by Society for Neuroscience

ARTICLE

Neurotransmitter- and neuromodulator-dependent alterations in phosphorylation of protein I in slices of rat facial nucleus

AC Dolphin and P Greengard

Protein I is a neuronal phosphoprotein associated primarily with synaptic vesicles. Regulation of its state of phosphorylation has been investigated in slices of rat facial nucleus. This brainstem motor nucleus has a facilitatory serotonergic input and contains no interneurons. Serotonin (5-hydroxytryptamine, 5-HT, 10(-4) M), in the presence of the phosphodiesterase inhibitor isobutylmethylxanthine (IBMX, 4 x 10(-5) M), converted approximately 26% of Protein I in these slices from the dephospho-form to the phospho-form. This effect was partially inhibited using two classical 5-HT antagonists, mianserin added to the slices during in vitro incubation and metergoline administered in vivo. The effect of 5-HT appeared to be Ca2+-dependent, unlike that of IBMX (10(-3) M). Adenosine, its analog 2- chloroadenosine, and ATP also increased the phosphorylation of Protein I in facial nucleus slices. 2-Chloroadenosine (5 x 10(-4) M) caused a 29% phosphorylation of Protein I, and this effect was not dependent on extracellular Ca2+. The phosphorylation of Protein I caused both by 2- chloroadenosine and by ATP was inhibited by the adenosine antagonist 2'- deoxyadenosine. Results of additional experiments suggest that the great majority of the Protein I in the facial nucleus is present in presynaptic terminals other than the serotonergic afferents. It is concluded that the stimulation by 5-HT and adenosine of Protein I phosphorylation results largely from a direct action of these compounds on those Protein I-containing terminals.

This article has been cited by other articles:

				E. Nestler, M Zata, and P Greengard A diurnal rhythm in pineal protein 1 content mediated by beta-adrenergic neurotransmission Science, July 23, 1982; 217(4557): 357 - 359. [Abstract] [PDF]

				E. Nestler, T. Rainbow, B. McEwen, and P Greengard Corticosterone increases the amount of protein 1, a neuron-specific phosphoprotein, in rat hippocampus Science, June 5, 1981; 212(4499): 1162 - 1164. [Abstract] [PDF]

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