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Journal of Neuroscience, Vol 1, 300-307, Copyright © 1981 by Society for Neuroscience
Specific changes in rapidly transported proteins during regeneration of the goldfish optic nerve
LI Benowitz, VE Shashoua and MG Yoon
Double labeling methods were used to identify changes in the complement of
proteins synthesized in the retinal ganglion cells and transported down the
optic nerve during the process of axonal regeneration. Eight to 62 days
after goldfish underwent a unilateral optic nerve crush, one eye was
labeled with [3H]-, the other with [14C]proline. Control and regenerating
optic nerves were dissected out and homogenized together after 5 hr, a time
which allowed us to examine selectively membrane- bound components which
migrate in the rapid phase of axoplasmic transport. Proteins from the two
sides were so-purified and separated by sodium dodecyl
sulfate-polyacrylamide gel electrophoresis. Analysis of the 3H and 14C
incorporation patterns along the gels revealed a radical shift away from
the normal labeling spectrum during regeneration, with selective changes in
labeling at particular molecular weights varying over a 3-fold range. Eight
days after crushing the optic nerve, the greatest increases in labeling
were seen for material with apparent molecular weights of 24,000 to 27,000,
44,000, and 210,000 daltons. These peaks declined thereafter, and on days
29 to 39, the most prominent increases were at 110,000 to 140,000 daltons.
These studies indicate a continuously changing pattern in the synthesis
and/or degradation of proteins that are rapidly transported down the optic
nerve during regeneration and point to molecular species potential
significance in the establishment of the visual map upon the brain.
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