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Journal of Neuroscience, Vol 1, 419-426, Copyright © 1981 by Society for Neuroscience
Characteristics of growth-associated polypeptides in regenerating toad retinal ganglion cell axons
JH Skene and M Willard
We report here certain properties of three polypeptides that are rapidly
transported in greatly increased amounts during the regeneration of toad
optic nerves. All three of these growth-associated polypeptides (GAPs) are
associated with a membrane(s) with a buoyant density similar to the plasma
membrane. Since none of these GAPs are solubilized substantially by washing
the membranes in either low or high ionic strength media, they may be
"integral membrane proteins. All three GAPs contain a significant number of
polar amino acids (judging from their isoelectric points and their rapid
electrophoretic migration under their native charge), suggesting that
portions of each molecule must extent away from the membrane surfaces. One
of these proteins (GAP- 50) is a fucosylated glycoprotein. GAP-50 and
GAP-43 appear to be transported preferentially to axon tips; GAP-24 may be
distributed more uniformly along the axons. GAP-24 has an extremely short
half-life (4 to 6 hr), while the maximum half-lives of GAP-43 (2 to 3 days)
and GAP- 50 (approximately 1 day) are longer. We discuss the relevance of
these properties to possible roles for these polypeptides in the control of
nerve regeneration.
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