 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 1, 777-783, Copyright © 1981 by Society for Neuroscience
Localization of sodium channels in cultured neural cells
WA Catterall
Sodium channels in cultured neural cells were localized by light
microscopic autoradiography of specifically bound 125I-scorpion toxin.
Ninety percent of the cell-bound 125I-scorpion toxin was associated
specifically with sodium channels as assessed by the blocking of
autoradiographic labeling by unlabeled scorpion toxin and by
depolarization. Sodium channels were distributed uniformly in the surface
membrane of neurites and cell bodies of both morphologically differentiated
and undifferentiated cells of clone N18 of mouse neuroblastoma C1300.
Sodium channels were distributed nonuniformly in many cultured spinal cord
neurons. Visual observation indicated that 37 +/- 5% of cultured spinal
cord neurons had a higher sodium channel density on the initial segment of
one or more neurites than on the cell body. For these neurons, the density
on one neurite initial segment averaged 7.4 +/- 1.9-fold greater than on
the adjacent cell body. This increased sodium channel density may be the
basis of the lower threshold for action potential generation at the axon
initial segment of motor neurons and some spinal interneurons in vivo.
This article has been cited by other articles:
|
|
|
|
 
M. Royeck, M.-T. Horstmann, S. Remy, M. Reitze, Y. Yaari, and H. Beck
Role of Axonal NaV1.6 Sodium Channels in Action Potential Initiation of CA1 Pyramidal Neurons
J Neurophysiol,
October 1, 2008;
100(4):
2361 - 2380.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P.-M. Martin, M. Carnaud, G. G. del Cano, M. Irondelle, T. Irinopoulou, J.-A. Girault, B. Dargent, and L. Goutebroze
Schwannomin-Interacting Protein-1 Isoform IQCJ-SCHIP-1 Is a Late Component of Nodes of Ranvier and Axon Initial Segments
J. Neurosci.,
June 11, 2008;
28(24):
6111 - 6117.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. F. Donnelly
Spontaneous action potential generation due to persistent sodium channel currents in simulated carotid body afferent fibers
J Appl Physiol,
May 1, 2008;
104(5):
1394 - 1401.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Dzhashiashvili, Y. Zhang, J. Galinska, I. Lam, M. Grumet, and J. L. Salzer
Nodes of Ranvier and axon initial segments are ankyrin G-dependent domains that assemble by distinct mechanisms
J. Cell Biol.,
June 21, 2007;
177(5):
857 - 870.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. B. Rasmussen, C. Frokjaer-Jensen, C. S. Jensen, H. S. Jensen, N. K. Jorgensen, H. Misonou, J. S. Trimmer, S.-P. Olesen, and N. Schmitt
Requirement of subunit co-assembly and ankyrin-G for M-channel localization at the axon initial segment
J. Cell Sci.,
March 15, 2007;
120(6):
953 - 963.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. J. Tehovnik, A. S. Tolias, F. Sultan, W. M. Slocum, and N. K. Logothetis
Direct and Indirect Activation of Cortical Neurons by Electrical Microstimulation
J Neurophysiol,
August 1, 2006;
96(2):
512 - 521.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Astman, M. J. Gutnick, and I. A. Fleidervish
Persistent sodium current in layer 5 neocortical neurons is primarily generated in the proximal axon.
J. Neurosci.,
March 29, 2006;
26(13):
3465 - 3473.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Boiko, A. Van Wart, J. H. Caldwell, S. R. Levinson, J. S. Trimmer, and G. Matthews
Functional Specialization of the Axon Initial Segment by Isoform-Specific Sodium Channel Targeting
J. Neurosci.,
March 15, 2003;
23(6):
2306 - 2313.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. C. McIntyre and W. M. Grill
Extracellular Stimulation of Central Neurons: Influence of Stimulus Waveform and Frequency on Neuronal Output
J Neurophysiol,
October 1, 2002;
88(4):
1592 - 1604.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Mathis, N. Denisenko-Nehrbass, J.-A. Girault, and E. Borrelli
Essential role of oligodendrocytes in the formation and maintenance of central nervous system nodal regions
Development,
December 1, 2001;
128(23):
4881 - 4890.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. Bennett and A. J. Baines
Spectrin and Ankyrin-Based Pathways: Metazoan Inventions for Integrating Cells Into Tissues
Physiol Rev,
July 1, 2001;
81(3):
1353 - 1392.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Rekling, G. D. Funk, D. A. Bayliss, X.-W. Dong, and J. L. Feldman
Synaptic Control of Motoneuronal Excitability
Physiol Rev,
April 1, 2000;
80(2):
767 - 852.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Srinivasan, M. Schachner, and W. A. Catterall
Interaction of voltage-gated sodium channels with the extracellular matrix molecules tenascin-C and tenascin-R
PNAS,
December 22, 1998;
95(26):
15753 - 15757.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Zhou, S. Lambert, P. L. Malen, S. Carpenter, L. M. Boland, and V. Bennett
AnkyrinG Is Required for Clustering of Voltage-gated Na Channels at Axon Initial Segments and for Normal Action Potential Firing
J. Cell Biol.,
November 30, 1998;
143(5):
1295 - 1304.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Waxman and J. Ritchie
Organization of ion channels in the myelinated nerve fiber
Science,
June 28, 1985;
228(4707):
1502 - 1507.
[Abstract]
[PDF]
|
|
|
|
|
|
|
W. Catterall
The molecular basis of neuronal excitability
Science,
February 17, 1984;
223(4637):
653 - 661.
[Abstract]
[PDF]
|
|
|
|
|
|
|
L.C. Fritz, H.-P.H. Moore, M.A. Raftery, and J.P. Brockes
Immunochemical Studies of the Voltage-sensitive Sodium Channel from the Electroplax of the Eel Electrophorus electricus
Cold Spring Harb Symp Quant Biol,
January 1, 1983;
48(0):
181 - 185.
[Abstract]
[PDF]
|
|
|
|
