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Journal of Neuroscience, Vol 10, 11-19, Copyright © 1990 by Society for Neuroscience
ATP-activated channels in rat and bullfrog sensory neurons: current- voltage relation and single-channel behavior
BP Bean, CA Williams and PW Ceelen
Department of Neurobiology, Harvard Medical School, Boston, Massachusetts 02115.
Ionic currents activated by extracellular adenosine 5'-triphosphate (ATP)
were studied in voltage-clamped dorsal root ganglion neurons from rats and
bullfrogs. Under quasiphysiological ionic conditions, ATP- activated
current reversed near 0 mV and showed strong inward rectification. Strong
inward rectification was maintained even in symmetric solutions of
divalent-free Cs glutamate. Examined with a resolution of 10s of
microseconds, the rectification was instantaneous. Inward current was
greatly reduced when N-methyl-D-glucamine was substituted for external Na.
ATP-activated inward currents could be recorded with Ca as the only
external cation; estimated from reversal potentials, the ratio of Ca to Na
permeability is about 0.3. Unitary channel activity could be recorded when
ATP was applied to outside-out patches. When activated, a single channel
flickered rapidly, with a mean current of about 0.5 pA at -100 mV. Large
concentrations of ATP put the channel in the activated, flickery condition
virtually all the time, while at lower concentrations, periods of
flickering were interspersed with closures. Analysis of whole-cell current
fluctuations showed precisely the characteristics expected if such channels
underlie the macroscopic currents.
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