 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 10, 125-135, Copyright © 1990 by Society for Neuroscience
NMDA receptors mediate poly- and monosynaptic potentials in motoneurons of rat embryos
L Ziskind-Conhaim
Department of Physiology, University of Wisconsin, Madison 53706.
We determined the contribution of glutamate receptor subtypes to developing
excitatory synaptic transmission in isolated spinal cord of rat embryos.
Using electrophysiological and morphological techniques, we studied the
pattern of development of synapses between dorsal root afferents and
motoneurons in lumbar spinal cords of 15- to 21-d-old rat embryos.
Motoneuron dendritic fields and afferent projections onto motoneurons were
identified by labeling with HRP. Afferents first entered the gray matter at
Day 15 of gestation, and by Day 16 they terminated close to motoneuron
dendritic trees. Afferent axons projected onto motoneuron dendritic fields
at Day 17, when boutons were detected on motoneuron dendrites that were
crossed by afferent axons. To determine the time course of formation of
functional sensorimotor synapses and their pharmacological properties, a
dorsal root was stimulated while recording intracellularly from segmental
motoneurons. At Day 16, excitatory postsynaptic potentials (EPSPs) with
long latencies, slow rates of rise, and long durations were recorded. The
amplitudes of these EPSPs increased with membrane depolarization and in the
absence of extracellular Mg2+. These EPSPs were blocked by D-2-
amino-5-phosphonovalerate (APV) and ketamine, which are selective
antagonists of N-methyl-D-aspartate (NMDA) receptors. These findings
suggest that initial synaptic transmission in embryonic motoneurons is
mediated solely by NMDA receptors. Short-latency EPSPs with fast rates of
rise were first recorded in most motoneurons by Day 17. These EPSPs were
composed of fast- and slow-rising potentials. The slow component was
blocked by APV, while the fast component was eliminated by 6-cyano-
7-nitroquinoxaline-2,3-dione and kynurenate. This indicates that the
short-latency EPSPs are mediated by both NMDA and non-NMDA receptors.
Dose-response curves of motoneurons to L-glutamate, NMDA, and kainate
demonstrated that motoneurons are sensitive to these agonists prior to the
formation of synapses between afferents and motoneurons. Motoneuron
responses to NMDA and kainate increased immediately after the onset of
short-latency EPSPs. This increased sensitivity could be due to
extracellular factors influenced by growing sensory axons or intrinsic
properties of differentiating motoneurons.
This article has been cited by other articles:
|
|
|
|
 
S. Tazerart, J.-C. Viemari, P. Darbon, L. Vinay, and F. Brocard
Contribution of Persistent Sodium Current to Locomotor Pattern Generation in Neonatal Rats
J Neurophysiol,
August 1, 2007;
98(2):
613 - 628.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Z. Mentis, E. Diaz, L. B. Moran, and R. Navarrete
Early alterations in the electrophysiological properties of rat spinal motoneurones following neonatal axotomy
J. Physiol.,
August 1, 2007;
582(3):
1141 - 1161.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Petruska, R. M. Ichiyama, D. L. Jindrich, E. D. Crown, K. E. Tansey, R. R. Roy, V. R. Edgerton, and L. M. Mendell
Changes in Motoneuron Properties and Synaptic Inputs Related to Step Training after Spinal Cord Transection in Rats
J. Neurosci.,
April 18, 2007;
27(16):
4460 - 4471.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. A. Hinckley and L. Ziskind-Conhaim
Electrical Coupling between Locomotor-Related Excitatory Interneurons in the Mammalian Spinal Cord.
J. Neurosci.,
August 15, 2006;
26(33):
8477 - 8483.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Ziskind-Conhaim and S. Redman
Spatiotemporal Patterns of Dorsal Root-Evoked Network Activity in the Neonatal Rat Spinal Cord: Optical and Intracellular Recordings
J Neurophysiol,
September 1, 2005;
94(3):
1952 - 1961.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. L. Arvanian, W. J. Bowers, J. C. Petruska, V. Motin, H. Manuzon, W. C. Narrow, H. J. Federoff, and L. M. Mendell
Viral Delivery of NR2D Subunits Reduces Mg2+ Block of NMDA Receptor and Restores NT-3-Induced Potentiation of AMPA-Kainate Responses in Maturing Rat Motoneurons
J Neurophysiol,
October 1, 2004;
92(4):
2394 - 2404.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. L. Arvanian, P. J. Horner, F. H. Gage, and L. M. Mendell
Chronic Neurotrophin-3 Strengthens Synaptic Connections to Motoneurons in the Neonatal Rat
J. Neurosci.,
September 24, 2003;
23(25):
8706 - 8712.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Ren and J. J. Greer
Ontogeny of Rhythmic Motor Patterns Generated in the Embryonic Rat Spinal Cord
J Neurophysiol,
March 1, 2003;
89(3):
1187 - 1195.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Ziskind-Conhaim, B.-X. Gao, and C. Hinckley
Ethanol Dual Modulatory Actions on Spontaneous Postsynaptic Currents in Spinal Motoneurons
J Neurophysiol,
February 1, 2003;
89(2):
806 - 813.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li and R. E. Burke
Developmental Changes in Short-Term Synaptic Depression in the Neonatal Mouse Spinal Cord
J Neurophysiol,
December 1, 2002;
88(6):
3218 - 3231.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Z Mentis, E. Diaz, L. B Moran, and R. Navarrete
Increased incidence of gap junctional coupling between spinal motoneurones following transient blockade of NMDA receptors in neonatal rats
J. Physiol.,
November 1, 2002;
544(3):
757 - 764.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Demir, B.-X. Gao, M. B. Jackson, and L. Ziskind-Conhaim
Interactions Between Multiple Rhythm Generators Produce Complex Patterns of Oscillation in the Developing Rat Spinal Cord
J Neurophysiol,
February 1, 2002;
87(2):
1094 - 1105.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. L. Arvanian and L. M. Mendell
Removal of NMDA Receptor Mg2+ Block Extends the Action of NT-3 on Synaptic Transmission in Neonatal Rat Motoneurons
J Neurophysiol,
July 1, 2001;
86(1):
123 - 129.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-X. Gao, C. Stricker, and L. Ziskind-Conhaim
Transition From GABAergic to Glycinergic Synaptic Transmission in Newly Formed Spinal Networks
J Neurophysiol,
July 1, 2001;
86(1):
492 - 502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Li and R. E. Burke
Short-Term Synaptic Depression in the Neonatal Mouse Spinal Cord: Effects of Calcium and Temperature
J Neurophysiol,
May 1, 2001;
85(5):
2047 - 2062.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. A. Nunez-Abades, J. M. Pattillo, T. M. Hodgson, and W. E. Cameron
Role of Synaptic Inputs in Determining Input Resistance of Developing Brain Stem Motoneurons
J Neurophysiol,
November 1, 2000;
84(5):
2317 - 2329.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. L. Arvanov, B. S. Seebach, and L. M. Mendell
NT-3 Evokes an LTP-Like Facilitation of AMPA/Kainate Receptor-Mediated Synaptic Transmission in the Neonatal Rat Spinal Cord
J Neurophysiol,
August 1, 2000;
84(2):
752 - 758.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. C. Rekling, G. D. Funk, D. A. Bayliss, X.-W. Dong, and J. L. Feldman
Synaptic Control of Motoneuronal Excitability
Physiol Rev,
April 1, 2000;
80(2):
767 - 852.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Metzger, A. Kulik, M. Sendtner, and K. Ballanyi
Contribution of Ca2+-Permeable AMPA/KA Receptors to Glutamate-Induced Ca2+ Rise in Embryonic Lumbar Motoneurons In Situ
J Neurophysiol,
January 1, 2000;
83(1):
50 - 59.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Rohrbough and N. C. Spitzer
Ca2+-Permeable AMPA Receptors and Spontaneous Presynaptic Transmitter Release at Developing Excitatory Spinal Synapses
J. Neurosci.,
October 1, 1999;
19(19):
8528 - 8541.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. S. Seebach, V. Arvanov, and L. M. Mendell
Effects of BDNF and NT-3 on Development of Ia/Motoneuron Functional Connectivity in Neonatal Rats
J Neurophysiol,
May 1, 1999;
81(5):
2398 - 2405.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-X. Gao and L. Ziskind-Conhaim
Development of Ionic Currents Underlying Changes in Action Potential Waveforms in Rat Spinal Motoneurons
J Neurophysiol,
December 1, 1998;
80(6):
3047 - 3061.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B.-X. Gao, G. Cheng, and L. Ziskind-Conhaim
Development of Spontaneous Synaptic Transmission in the Rat Spinal Cord
J Neurophysiol,
May 1, 1998;
79(5):
2277 - 2287.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Metzger, S. Wiese, and M. Sendtner
Effect of Glutamate on Dendritic Growth in Embryonic Rat Motoneurons
J. Neurosci.,
March 1, 1998;
18(5):
1735 - 1742.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Sato, Y. Momose-Sato, A. Hirota, T. Sakai, and K. Kamino
Optical Mapping of Neural Responses in the Embryonic Rat Brainstem with Reference to the Early Functional Organization of Vagal Nuclei
J. Neurosci.,
February 15, 1998;
18(4):
1345 - 1362.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Kalb
Regulation of motor neuron dendrite growth by NMDA receptor activation
Development,
January 11, 1994;
120(11):
3063 - 3071.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M Fitzgerald, G. Kwiat, J Middleton, and A Pini
Ventral spinal cord inhibition of neurite outgrowth from embryonic rat dorsal root ganglia
Development,
January 4, 1993;
117(4):
1377 - 1384.
[Abstract]
[PDF]
|
|
|
|
|
