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Journal of Neuroscience, Vol 10, 3413-3420, Copyright © 1990 by Society for Neuroscience
Calcium permeability of the nicotinic acetylcholine receptor: the single-channel calcium influx is significant
ER Decker and JA Dani
Department of Molecular Physiology and Biophysics, Baylor College of Medicine, Houston, Texas 77030.
The calcium permeability of the mouse muscle nicotinic ACh receptor (nAChR)
was determined using patch-clamp techniques. Single-channel currents were
measured in pure external calcium and in mixtures of calcium with cesium or
sodium. At low concentrations, calcium decreases the current carried by the
monovalent cation. At higher concentrations, calcium displaces the
monovalent cation as the main current carrier. In pure external calcium,
the conductance of the nAChR is similar to the conductance of the NMDA
receptor or the L-type Ca channel. With pure 110-mM calcium as the external
cation, the slope conductance of the nAChR channel at negative potentials
is 12 pS. An ion-permeation model based on the structure and function of
the channel describes the currents. The ion-permeation model predicts that
calcium contributes about 2% of the total inward current through a nAChR
channel in physiologic solution. The current is about 7% of the calcium
current through an L-type Ca channel. Because nAChRs are densely packed at
the neuromuscular end plate, the calcium influx at an active synapse is
expected to produce a locally high-calcium environment.
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