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Journal of Neuroscience, Vol 10, 3615-3626, Copyright © 1990 by Society for Neuroscience
Development of the retinotectal projection in zebrafish embryos under TTX-induced neural-impulse blockade
CA Stuermer, B Rohrer and H Munz
Friedrich-Miescher-Laboratorium der Max-Planck-Gesellschaft, Tubingen, Federal Republic of Germany.
The influence of neural activity on the morphology of retinal-axon-
terminal arbors and the precision of the developing retinotectal projection
in zebrafish embryos was explored. Terminal-arbor morphology and their
distribution in the tectum was determined with anatomical fiber-tracing
methods using the fluorescent dyes dil and diO. To allow development under
activity-deprived conditions, TTX was injected into the eyes of
30-38-hr-old zebrafish embryos at concentrations that effectively blocked
neural activity both in retinal ganglion cells and throughout the CNS. Much
like axons with normal neural-activity patterns, activity-deprived axons
from dorsal and ventral and from temporal and nasal regions in the retina
terminated over retinotopically appropriate and nonoverlapping regions of
the tectum. Even after ablation of 1 hemiretina at the time of axonal
outgrowth, activity-deprived axons from the remaining hemiretina grew
directed toward and arborized selectively within their retinotopically
appropriate tectal half in the same way as would nondeprived axons. Besides
being retinotopic, the area over which small populations of
activity-deprived axons from neighboring ganglion cells arborize is as
small as that of active axons. The size of terminal arbors of retinal
ganglion cell axons was unaffected by blockade of neural activity. The mean
terminal-arbor size was 27 x 18 microns for the TTX-injected and 31 x 22
microns for the control embryos. The tectal coverage of TTX- blocked and
control axons was equally small, with values of 1.4% and 1.6%,
respectively. These data show that a precisely organized retinotopic map in
developing zebrafish forms independent of neural- impulse activity.
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