 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 10, 3873-3879, Copyright © 1990 by Society for Neuroscience
The interactions between plasma membrane depolarization and glutamate receptor activation in the regulation of cytoplasmic free calcium in cultured cerebellar granule cells
MJ Courtney, JJ Lambert and DG Nicholls
Department of Biochemistry, University of Dundee, Scotland, U.K.
The complex modulation of cytoplasmic free calcium concentration ([Ca2+]c)
in primary cultures of cerebellar granule cells in response to glutamate
receptor agonists has been the subject of several contradictory reports. We
here show that 3 components of the [Ca2+]c response can be distinguished:
(1) Ca2+ entry through voltage-dependent Ca2+ channels, following KCl- or
receptor-evoked depolarization, (2) Ca2+ entry through NMDA receptor
channels, and (3) liberation of internal Ca2+ via a metabolotropic
receptor. Depolarization with KCl induced a transient [Ca2+]c response
(subject to voltage inactivation) decaying to a sustained plateau (largely
inhibited by nifedipine). The NMDA response was potentiated by glycine,
totally inhibited by (+)5-
methyl-10,11-dihydro-5H-dibenzo[a,d]cyclohepten-5,10-imine maleate (MK-
801), and blocked by Mg2+ in a voltage-sensitive manner. Polarized cells
displayed small responses to quisqualate (QA) and alpha-amino-3-
hydroxy-5-methyl-4-isoxazole-propionic acid (AMPA). Depolarization enhanced
a transient response to QA, but not to AMPA. Trans-1-amino-1,3-
cyclopentanedicarboxylic acid (trans-ACPD), a selective agonist for the
metabolotropic glutamate receptor, caused a transient elevation of [Ca2+]c,
which was blocked by prior exposure to QA but not AMPA. The prolonged
[Ca2+]c response to kainate (KA) can be resolved into 2 major components:
an indirect NMDA receptor-mediated response due to released glutamate and a
nifedipine-sensitive component consistent with depolarization-mediated
entry via Ca2+ channels. 6-Cyano-7- nitroquinoxaline-2,3-dione (CNQX), QA
at greater than 10 microM, and AMPA (but not trans-ACPD) reversed the KA
response, consistent with an inactivation of the KA receptor.(ABSTRACT
TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
|
|
 
N. Yadava and D. G. Nicholls
Spare Respiratory Capacity Rather Than Oxidative Stress Regulates Glutamate Excitotoxicity after Partial Respiratory Inhibition of Mitochondrial Complex I with Rotenone
J. Neurosci.,
July 4, 2007;
27(27):
7310 - 7317.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Qi, N. R. Stephens, and E. P. Spalding
Calcium Entry Mediated by GLR3.3, an Arabidopsis Glutamate Receptor with a Broad Agonist Profile
Plant Physiology,
November 1, 2006;
142(3):
963 - 971.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Vesce, M. B. Jekabsons, L. I. Johnson-Cadwell, and D. G. Nicholls
Acute Glutathione Depletion Restricts Mitochondrial ATP Export in Cerebellar Granule Neurons
J. Biol. Chem.,
November 18, 2005;
280(46):
38720 - 38728.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. E. Kushnareva, S. E. Wiley, M. W. Ward, A. Y. Andreyev, and A. N. Murphy
Excitotoxic Injury to Mitochondria Isolated from Cultured Neurons
J. Biol. Chem.,
August 12, 2005;
280(32):
28894 - 28902.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Cao, M. M. Semenova, V. T. Solovyan, J. Han, E. T. Coffey, and M. J. Courtney
Distinct Requirements for p38{alpha} and c-Jun N-terminal Kinase Stress-activated Protein Kinases in Different Forms of Apoptotic Neuronal Death
J. Biol. Chem.,
August 20, 2004;
279(34):
35903 - 35913.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Jekabsons and D. G. Nicholls
In Situ Respiration and Bioenergetic Status of Mitochondria in Primary Cerebellar Granule Neuronal Cultures Exposed Continuously to Glutamate
J. Biol. Chem.,
July 30, 2004;
279(31):
32989 - 33000.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Spruce, L. A. Campbell, N. McTavish, M. A. Cooper, M. V. L. Appleyard, M. O'Neill, J. Howie, J. Samson, S. Watt, K. Murray, et al.
Small Molecule Antagonists of the {sigma}-1 Receptor Cause Selective Release of the Death Program in Tumor and Self-Reliant Cells and Inhibit Tumor Growth in Vitro and in Vivo
Cancer Res.,
July 15, 2004;
64(14):
4875 - 4886.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Schrader, S. P. Perrett, L. Ye, and M. J. Friedlander
Substrates for Coincidence Detection and Calcium Signaling for Induction of Synaptic Potentiation in the Neonatal Visual Cortex
J Neurophysiol,
June 1, 2004;
91(6):
2747 - 2764.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Rego, M. W. Ward, and D. G. Nicholls
Mitochondria Control AMPA/Kainate Receptor-Induced Cytoplasmic Calcium Deregulation in Rat Cerebellar Granule Cells
J. Neurosci.,
March 15, 2001;
21(6):
1893 - 1901.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. T. Coffey, V. Hongisto, M. Dickens, R. J. Davis, and M. J. Courtney
Dual Roles for c-Jun N-Terminal Kinase in Developmental and Stress Responses in Cerebellar Granule Neurons
J. Neurosci.,
October 15, 2000;
20(20):
7602 - 7613.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. W. Ward, A. C. Rego, B. G. Frenguelli, and D. G. Nicholls
Mitochondrial Membrane Potential and Glutamate Excitotoxicity in Cultured Cerebellar Granule Cells
J. Neurosci.,
October 1, 2000;
20(19):
7208 - 7219.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Tuittila, M. G. Santagati, M. Röyttä, J. A. Määttä, and A. E. Hinkkanen
Replicase Complex Genes of Semliki Forest Virus Confer Lethal Neurovirulence
J. Virol.,
May 15, 2000;
74(10):
4579 - 4589.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
D. G. Nicholls and S. L. Budd
Mitochondria and Neuronal Survival
Physiol Rev,
January 1, 2000;
80(1):
315 - 360.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Netzeband, S. M. Conroy, K. L. Parsons, and D. L. Gruol
Cannabinoids Enhance NMDA-Elicited Ca2+ Signals in Cerebellar Granule Neurons in Culture
J. Neurosci.,
October 15, 1999;
19(20):
8765 - 8777.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M.-L. Wu, J.-H. Chen, W.-H. Chen, Y.-J. Chen, and K.-C. Chu
Novel role of the Ca2+-ATPase in NMDA-induced intracellular acidification
Am J Physiol Cell Physiol,
October 1, 1999;
277(4):
C717 - C727.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. F. Castilho, O. Hansson, M. W. Ward, S. L. Budd, and D. G. Nicholls
Mitochondrial Control of Acute Glutamate Excitotoxicity in Cultured Cerebellar Granule Cells
J. Neurosci.,
December 15, 1998;
18(24):
10277 - 10286.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Pinto, S. Gillard, F. Moss, K. Whyte, P. Brust, M. Williams, K. Stauderman, M. Harpold, B. Lang, J. Newsom-Davis, et al.
Human autoantibodies specific for the alpha 1A calcium channel subunit reduce both P-type and Q-type calcium currents in cerebellar neurons
PNAS,
July 7, 1998;
95(14):
8328 - 8333.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Belelli, J. J. Lambert, J. A. Peters, K. Wafford, and P. J. Whiting
The interaction of the general anesthetic etomidate with the gamma -aminobutyric acid type A receptor is influenced by a single amino acid
PNAS,
September 30, 1997;
94(20):
11031 - 11036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Courtney, K. E. O. Akerman, and E. T. Coffey
Neurotrophins Protect Cultured Cerebellar Granule Neurons against the Early Phase of Cell Death by a TwoComponent Mechanism
J. Neurosci.,
June 1, 1997;
17(11):
4201 - 4211.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H Komuro and P Rakic
Selective role of N-type calcium channels in neuronal migration
Science,
August 7, 1992;
257(5071):
806 - 809.
[Abstract]
[PDF]
|
|
|
|
