QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Naegele, J. R. Articles by Katz, L. C. Search for Related Content PubMed PubMed Citation Articles by Naegele, J. R. Articles by Katz, L. C. Pubmed/NCBI databases Compound via MeSH Substance via MeSH

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 10, 540-557, Copyright © 1990 by Society for Neuroscience

ARTICLE

Cell surface molecules containing N-acetylgalactosamine are associated with basket cells and neurogliaform cells in cat visual cortex

JR Naegele and LC Katz
Laboratory of Neurobiology, Rockefeller University, New York, New York.

In the cerebral cortex, the plant lectin Vicia villosa (VVA) selectively stains the surfaces of nonpyramidal neurons. This lectin binds specifically to alpha- and beta-linked N-acetylgalactosamine (GalNac). VVA-reactive carbohydrate is highly concentrated in layer 4 of the primary visual cortex of the cat, where it is associated exclusively with GABAergic local circuit neurons. We have studied this neuronal subset with intracellular electrophysiological recording and dye marking to identify the particular cell types expressing surface GalNac. Five different types of local circuit neurons were stained intracellularly (N = 45), but only 2 types, the columnar basket and large neurogliaform cells, were also labeled by the lectin (N = 19/45). Lectin negative types included small basket, chandelier, and large bitufted cells (26/45). Spiny stellate and pyramidal neurons were also lectin negative. Electrophysiological recordings revealed differences in the duration of action potentials in smooth versus spiny stellates but no differences between lectin-positive or -negative types. A biochemical analysis of cortical glycoproteins by SDS-PAGE and lectin blotting revealed multiple bands containing GalNac enriched in membrane fractions. These carbohydrate-containing molecules may be part of a biochemical mechanism for linking together cells with common functional properties.

This article has been cited by other articles:

				P. A. McRae, M. M. Rocco, G. Kelly, J. C. Brumberg, and R. T. Matthews Sensory Deprivation Alters Aggrecan and Perineuronal Net Expression in the Mouse Barrel Cortex J. Neurosci., May 16, 2007; 27(20): 5405 - 5413. [Abstract] [Full Text] [PDF]

				L. G. Nowak, R. Azouz, M. V. Sanchez-Vives, C. M. Gray, and D. A. McCormick Electrophysiological Classes of Cat Primary Visual Cortical Neurons In Vivo as Revealed by Quantitative Analyses J Neurophysiol, March 1, 2003; 89(3): 1541 - 1566. [Abstract] [Full Text] [PDF]

				M. Gur, A. Beylin, and D. M. Snodderly Physiological Properties of Macaque V1 Neurons are Correlated With Extracellular Spike Amplitude, Duration, and Polarity J Neurophysiol, September 1, 1999; 82(3): 1451 - 1464. [Abstract] [Full Text] [PDF]

				A. Inoue and J. R. Sanes Lamina-Specific Connectivity in the Brain: Regulation by N-Cadherin, Neurotrophins, and Glycoconjugates Science, May 30, 1997; 276(5317): 1428 - 1431. [Abstract] [Full Text]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help