 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 10, 684-692, Copyright © 1990 by Society for Neuroscience
Cortical radial glia: identification in tissue culture and evidence for their transformation to astrocytes
SM Culican, NL Baumrind, M Yamamoto and AL Pearlman
Department of Cell Biology, Washington University School of Medicine, St. Louis, Missouri 63110.
Radial glia are transiently present in the developing cerebral cortex,
where they are thought to guide the migration of neurons from the
proliferative zone to the forming cortical plate. To provide a framework
for experimental studies of radial glia, we have defined morphological and
immunocytochemical criteria to identify them in primary cultures of
cortical cells obtained at embryonic day 13 in the mouse. Cortical radial
glia in culture for 1-2 d resemble radial glia in vivo: they have a long,
thin, unbranched process extending from one or both ends of the elongated
cell body and are labeled with the monoclonal antibody RC1 but not with
antibodies to glial fibrillary acidic protein (abGFAP). We tested the
specificity of RC1 by double- labeling with a panel of cell-type specific
antibodies, and found that it labels radial glia, astrocytes, and
fibroblast-like cells, but not neurons. Fibroblasts are easily
distinguished from glia by morphology and by labeling with antibodies to
fibronectin. To test the hypothesis that radial glia become astrocytes when
their developmental role is complete, we examined their morphological and
immunocytochemical development in culture. After 3-4 d in vitro radial glia
develop several branched processes; in this transitional stage they are
labeled by both RC1 and abGFAP. Many radial glia lose RC1 immunoreactivity
as they become increasingly branched and immunoreactive to abGFAP. In areas
of the cultures that have few neurons and in cultures depleted of neurons
by washing, flat, nonprocess-bearing glia predominate. These cells do not
lose immunoreactivity to RC1 during the 9-d period of observation even
though they acquire GFAP.(ABSTRACT TRUNCATED AT 250 WORDS)
This article has been cited by other articles:
|
|
|
|
 
N. Kessaris, N. Pringle, and W. D Richardson
Specification of CNS glia from neural stem cells in the embryonic neuroepithelium
Phil Trans R Soc B,
January 12, 2008;
363(1489):
71 - 85.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. Kulbatski, A. J. Mothe, A. Keating, Y. Hakamata, E. Kobayashi, and C. H. Tator
Oligodendrocytes and Radial Glia Derived From Adult Rat Spinal Cord Progenitors: Morphological and Immunocytochemical Characterization
J. Histochem. Cytochem.,
March 1, 2007;
55(3):
209 - 222.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Gierdalski, S. P. Sardi, G. Corfas, and S. L. Juliano
Endogenous Neuregulin Restores Radial Glia in a (Ferret) Model of Cortical Dysplasia
J. Neurosci.,
September 14, 2005;
25(37):
8498 - 8504.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. A. Patten, J. M. Peyrin, G. Weinmaster, and G. Corfas
Sequential Signaling through Notch1 and erbB Receptors Mediates Radial Glia Differentiation
J. Neurosci.,
July 9, 2003;
23(14):
6132 - 6140.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Rakic
Developmental and Evolutionary Adaptations of Cortical Radial Glia
Cereb Cortex,
June 1, 2003;
13(6):
541 - 549.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. S. Schmid, B. McGrath, B. E. Berechid, B. Boyles, M. Marchionni, N. Sestan, and E. S. Anton
Neuregulin 1-erbB2 signaling is required for the establishment of radial glia and their transformation into astrocytes in cerebral cortex
PNAS,
April 1, 2003;
100(7):
4251 - 4256.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Wang, S. Andersson, M. Warner, and J.-A. Gustafsson
Estrogen receptor (ER)beta knockout mice reveal a role for ERbeta in migration of cortical neurons in the developing brain
PNAS,
January 21, 2003;
100(2):
703 - 708.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. P. Pringle, W.-P. Yu, M. Howell, J. S. Colvin, D. M. Ornitz, and W. D. Richardson
Fgfr3 expression by astrocytes and their precursors: evidence that astrocytes and oligodendrocytes originate in distinct neuroepithelial domains
Development,
January 1, 2003;
130(1):
93 - 102.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. B. Reid and C. A. Walsh
Evidence of Common Progenitors and Patterns of Dispersion in Rat Striatum and Cerebral Cortex
J. Neurosci.,
May 15, 2002;
22(10):
4002 - 4014.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P Malatesta, E Hartfuss, and M Gotz
Isolation of radial glial cells by fluorescent-activated cell sorting reveals a neuronal lineage
Development,
January 12, 2000;
127(24):
5253 - 5263.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. C. Noctor, S. L. Palmer, T. Hasling, and S. L. Juliano
Interference with the Development of Early Generated Neocortex Results in Disruption of Radial Glia and Abnormal Formation of Neocortical Layers
Cereb Cortex,
March 1, 1999;
9(2):
121 - 136.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. A. Mong, E. Glaser, and M. M. McCarthy
Gonadal Steroids Promote Glial Differentiation and Alter Neuronal Morphology in the Developing Hypothalamus in a Regionally Specific Manner
J. Neurosci.,
February 15, 1999;
19(4):
1464 - 1472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Golden and C. Cepko
Clones in the chick diencephalon contain multiple cell types and siblings are widely dispersed
Development,
January 1, 1996;
122(1):
65 - 78.
[Abstract]
[PDF]
|
|
|
|
|
|
|
E Noll and R. Miller
Oligodendrocyte precursors originate at the ventral ventricular zone dorsal to the ventral midline region in the embryonic rat spinal cord
Development,
January 6, 1993;
118(2):
563 - 573.
[Abstract]
[PDF]
|
|
|
|
|
