 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 10, 1070-1081, Copyright © 1990 by Society for Neuroscience
Autoradiographical detection of cholecystokinin-A receptors in primate brain using 125I-Bolton Hunter CCK-8 and 3H-MK-329
DR Hill, TM Shaw, W Graham and GN Woodruff
Merck Sharp and Dohme Research Laboratories, Neuroscience Research Centre, Harlow, Essex, United Kingdom.
In vitro autoradiography was performed in order to visualize
cholecystokinin-A (CCK-A) receptors in sections of Cynomolgus monkey brain.
CCK-A receptors were defined as those which displayed high affinity for the
selective non-peptide antagonist MK-329 (L-364,718) and were detected in
several regions by selective inhibition of 125I- Bolton Hunter CCK using
MK-329 or direct labeling with 3H-MK-329. In the caudal medulla, high
densities of CCK-A sites were present in the nucleus tractus solitarius,
especially the caudal and medial aspects, and also the dorsal motor nucleus
of the vagus. CCK-A sites were localized to a number of hypothalamic nuclei
such as the supraoptic and paraventricular nuclei, the dorsomedial and
infundibular nuclei as well as the neurohypophysis. The mammillary bodies
and supramammillary nuclei also contained CCK-A receptor sites. High
concentrations of CCK- A receptors were present in the substantia nigra
zona compacta and also the ventral tegmental area and may be associated
with dopamine cell bodies. Binding of 3H-MK-329 was also detected in parts
of the caudate nucleus and ventral putamen. The detection, by
autoradiographical means, of CCK-A receptors throughout the Cynomolgus
monkey brain contrasts with similar studies performed using rodents and
suggests differences in the density and, perhaps, the importance of CCK-A
receptors in the primate as opposed to the rodent. The data suggest the
possibility that CCK-A receptors may be involved in a number of important
brain functions as diverse as the processing of sensory information from
the gut, the regulation of hormone secretion, and the activity of dopamine
cell activity.
This article has been cited by other articles:
|
|
|
|
 
E. Anderzhanova, M. Covasa, and A. Hajnal
Altered basal and stimulated accumbens dopamine release in obese OLETF rats as a function of age and diabetic status
Am J Physiol Regulatory Integrative Comp Physiol,
August 1, 2007;
293(2):
R603 - R611.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Giacobini and S. Wray
Cholecystokinin Directly Inhibits Neuronal Activity of Primary Gonadotropin-Releasing Hormone Cells through Cholecystokinin-1 Receptor
Endocrinology,
January 1, 2007;
148(1):
63 - 71.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P.-Y. Deng and S. Lei
Bidirectional modulation of GABAergic transmission by cholecystokinin in hippocampal dentate gyrus granule cells of juvenile rats
J. Physiol.,
April 15, 2006;
572(2):
425 - 442.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Zheng, M. W. Lewis, and R. A. Travagli
In vitro analysis of the effects of cholecystokinin on rat brain stem motoneurons
Am J Physiol Gastrointest Liver Physiol,
May 1, 2005;
288(5):
G1066 - G1073.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Nomoto, M. Ohta, S. Kanai, Y. Yoshida, S. Takiguchi, A. Funakoshi, and K. Miyasaka
Absence of the cholecystokinin-A receptor deteriorates homeostasis of body temperature in response to changes in ambient temperature
Am J Physiol Regulatory Integrative Comp Physiol,
September 1, 2004;
287(3):
R556 - R561.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Noble, S. A. Wank, J. N. Crawley, J. Bradwejn, K. B. Seroogy, M. Hamon, and B. P. Roques
International Union of Pharmacology. XXI. Structure, Distribution, and Functions of Cholecystokinin Receptors
Pharmacol. Rev.,
December 1, 1999;
51(4):
745 - 781.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z.-B. You, T. M. Tzschentke, E. Brodin, and R. A. Wise
Electrical Stimulation of the Prefrontal Cortex Increases Cholecystokinin, Glutamate, and Dopamine Release in the Nucleus Accumbens: an In Vivo Microdialysis Study in Freely Moving Rats
J. Neurosci.,
August 15, 1998;
18(16):
6492 - 6500.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. Rady, W. Lin, and J. M. Fujimoto
Pentobarbital Antagonism of Morphine Analgesia Mediated by Spinal Cholecystokinin
J. Pharmacol. Exp. Ther.,
March 1, 1998;
284(3):
878 - 885.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
S. Takiguchi, Y. Takata, N. Takahashi, K. Kataoka, T. Hirashima, K. Kawano, K. Miyasaka, A. Funakoshi, and A. Kono
A disrupted cholecystokinin A receptor gene induces diabetes in obese rats synergistically with ODB1 gene
Am J Physiol Endocrinol Metab,
February 1, 1998;
274(2):
E265 - E270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. A. Fossa, M. J. Depasquale, J. Morrone, S. H. Zorn, D. Bryce, J. A. Lowe, and S. McLean
Cardiovascular Effects of Cholecystokinin-4 Are Mediated By the Cholecystokinin-B Receptor Subtype In the Conscious Guinea Pig and Dog
J. Pharmacol. Exp. Ther.,
April 1, 1997;
281(1):
180 - 187.
[Abstract]
[Full Text]
|
|
|
|
