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Journal of Neuroscience, Vol 10, 1779-1787, Copyright © 1990 by Society for Neuroscience
Neuronal properties and trophic activities of immortalized hippocampal cells from embryonic and young adult mice
HJ Lee, DN Hammond, TH Large, JD Roback, JA Sim, DA Brown, UH Otten and BH Wainer
Committee on Neurobiology, University of Chicago, Illinois 60637.
The hippocampal formation elaborates trophic factors such as nerve growth
factor (NGF) to support the cholinergic innervation it receives from the
septal region. To further study the trophic interactions of this pathway,
hippocampal cells from embryonic day 18 and postnatal day 21 mice were
immortalized via somatic cell fusion to N18TG2 neuroblastoma cells. The
hippocampal cell lines exhibit morphological and cytoskeletal features
which are typical of their neuronal parents but which are not expressed by
the neuroblastoma parent. When differentiated with retinoic acid, the
hippocampal cell lines exhibit electrophysiological features similar to
cultured hippocampal neurons. Many of the lines constitutively express high
levels of NGF, and at least one cell line exerts a non-NGF trophic effect
on the expression of choline acetyltransferase by septal neurons in vitro.
These cell lines are potentially useful for investigating the neurochemical
and excitable properties of hippocampal neurons and identifying novel
trophic activities that promote the development and maintenance of the
septohippocampal pathway.
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