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Journal of Neuroscience, Vol 10, 2338-2351, Copyright © 1990 by Society for Neuroscience
Membrane properties and discharge characteristics of guinea pig dorsal cochlear nucleus neurons studied in vitro
PB Manis
Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
Intracellular recordings were made from neurons of the guinea pig dorsal
cochlear nucleus in an in vitro brain slice preparation. The membrane
properties of the cells were studied, and the membrane potentials were
manipulated by current injection to determine how intrinsic conductances
might alter the cell discharge patterns. Eleven cells were marked with
Lucifer yellow. Ten of these cells were identified as the large pyramidal
cells of layer 2 of this nucleus, and 1 cell was identified as a "vertical"
cell in layer 3. Two kinds of action potentials were observed: simple
spikes and complex spikes. This report discusses only cells with simple
spikes. Simple spiking cells (60/72 recorded cells; all stained cells were
simple spiking cells) discharged in a regular fashion with depolarization,
and had linear frequency-current relationships up to 2 nA with a mean slope
of 116 Hz/nA. The discharge rate was approximately constant throughout the
current pulse. Responses of simple spiking cells to depolarizing current
steps superimposed on a steady-state membrane hyperpolarization were
studied. When the membrane has been held hyperpolarized, small current
pulses produce a long-latency regular train of action potentials. Larger
current pulses superimposed on membrane hyperpolarization can produce a
short-latency action potential followed by a long silent interval (i.e., a
long first interspike interval), and finally a regular train of spikes. It
is concluded that the membrane conductances of DCN pyramidal cells are
capable of generating at least 3 discharge patterns (regular firing, long
first spike latency, and long first interspike interval) depending on the
state of the membrane potential prior to a depolarizing current step. These
responses are similar to the "chopper," "buildup," and "pauser" discharge
patterns reported for these cells in vivo in response to tone bursts. The
modulation of the intrinsic membrane conductances by membrane polarization
and the possible contribution of these conductances to the generation of
DCN discharge patterns provide new insights into the mechanisms underlying
the responses of DCN cells to acoustic stimuli.
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