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Journal of Neuroscience, Vol 10, 2513-2527, Copyright © 1990 by Society for Neuroscience
Immunocytochemical localization of the neuron-specific form of the c- src gene product, pp60c-src(+), in rat brain
MM Sugrue, JS Brugge, DR Marshak, P Greengard and EL Gustafson
Department of Microbiology, SUNY, Stony Brook 11794.
Neurons express high levels of a variant form of the c-src gene product,
denoted pp60c-src(+), which contains a 6 amino acid insert in the
amino-terminal half of the c-src protein. We have determined the
localization of pp60c-src(+) in neurons using an affinity-purified anti-
peptide antibody, referred to as affi-SB12, that exclusively recognizes
this neuron-specific form of the c-src gene product. Using affi-SB12, we
examined the distribution of pp60c-src(+) by immunoperoxidase staining of
sections through adult rat brains, pp60c-src(+) was widely distributed in
rat brain and appeared to be differentially expressed in subpopulations of
neurons. The majority of immunoreactive neurons was found in the
mesencephalon, cerebellum, pons, and medulla. Telencephalic structures that
contained substantial populations of pp60c-src(+)-immunoreactive neurons
included layer V of the cerebral cortex and the ventral pallidum. Within
individual neurons, pp60c- src(+) immunoreactivity was localized to the
cell soma and dendritic processes, while labeling of axons and nerve
terminals (puncta) was not as readily detected. Dense accumulations of
immunoreactive axons were rare, being most prominent in portions of the
inferior and superior olive, and in the spinal trigeminal nucleus. While
the regional distribution of pp60c-src(+) immunoreactivity does not
correlate with any specific neuronal cell type or first messenger system,
this unique pattern of expression of pp60c-src(+) suggests the existence of
a previously uncharacterized functional organization within the brain.
Furthermore, the localization of this neuron-specific tyrosine kinase in
functionally important areas of the nerve cell, namely, dendritic
processes, axons, and nerve terminals, suggests that pp60c-src(+) may
regulate pleiotropic functions in specific classes of neurons in the adult
central nervous system.
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