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Journal of Neuroscience, Vol 10, 2587-2600, Copyright © 1990 by Society for Neuroscience
Localization of dopamine D2 receptor mRNA and D1 and D2 receptor binding in the rat brain and pituitary: an in situ hybridization- receptor autoradiographic analysis
A Mansour, JH Meador-Woodruff, JR Bunzow, O Civelli, H Akil and SJ Watson
Mental Health Research Institute, University of Michigan, Ann Arbor 48109-0720.
Several lines of evidence suggest the existence of multiple dopamine
receptor subtypes, referred to as D1 and D2. The present study examines the
distribution of these dopamine binding sites in the rat brain and pituitary
in relation to the distribution of D2 receptor mRNA using a combination of
in vitro receptor autoradiographic and in situ hybridization techniques.
3H-Raclopride and 3H-SCH23390 (in the presence of 1 microM ketanserin) were
used to label D2 and D1 receptor binding sites, respectively, while a 495
bp cRNA probe synthesized from the Sac I-Bgl II fragment of a rat D2
receptor cDNA was used to visualize the D2 receptor mRNA. Analysis of
adjacent tissue sections in which receptor autoradiography and in situ
hybridization had been performed revealed several brain regions where the
D2 binding site and corresponding mRNA appear to be similarly distributed,
including the caudate-putamen, nucleus accumbens, olfactory tubercle,
globus pallidus, substantia nigra, and ventral tegmental area. In the
pituitary gland, D2 binding sites and mRNA appear to be codistributed with
very dense levels in the intermediate lobe and individually labeled cells
in the anterior lobe. Brain regions demonstrating a lack of correspondence
between the distribution of the D2 binding site and D2 receptor mRNA
include the olfactory bulb, neocortex, paleocortex, hippocampus, and zona
incerta. Several hypotheses are discussed to explain the lack of
correspondence in certain brain regions; these include the localization of
receptor binding sites on both fibers and cell bodies and receptor
transport. These studies provide a better understanding of the anatomical
distribution of the D2 receptor and serve as a framework for future
regulatory and anatomical mapping studies. By focusing on specific brain
regions, such as the nigrostriatal system, hippocampus, and olfactory bulb,
they provide insights into D2 receptor synthesis, transport, and insertion
into cell membranes.
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