QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pollock, J. D. Articles by Rudy, B. Search for Related Content PubMed PubMed Citation Articles by Pollock, J. D. Articles by Rudy, B.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 10, 2626-2637, Copyright © 1990 by Society for Neuroscience

ARTICLE

Differential effects of NGF, FGF, EGF, cAMP, and dexamethasone on neurite outgrowth and sodium channel expression in PC12 cells

JD Pollock, M Krempin and B Rudy
Division of Biology, California Institute of Technology, Pasadena 91125.

PC12 cells are a pheochromocytoma cell line that can be made to differentiate into sympatheticlike neurons by nerve growth factor (NGF). An essential component of the NGF-induced differentiation is the development of action potentials and sodium channels. Using whole-cell clamp we have confirmed that NGF produces a 5- to 6-fold increase in sodium channel density. The sodium channels induced by NGF are not different from those in cells not treated with NGF and are similar to those in other cell types. Basic fibroblast growth factor (FGF), another growth factor that causes PC12 cells to differentiate into sympathetic-like neurons, also produces a 5- to 6-fold increase in sodium current density with channels indistinguishable from those in PC12 cells treated and not treated with NGF. Basic FGF produces the same or somewhat larger increase in sodium channel density but much less neurite outgrowth. In contrast, epidermal growth factor does not produce neurite outgrowth but induces a small, reproducible increase in sodium channel density. Cyclic AMP produces spike-like processes but not neurites and results in a decrease in sodium current and sodium current density. Dexamethasone, a synthetic glucocorticoid, inhibits the increase in sodium current and sodium current density but does not antagonize the neurite outgrowth induced by NGF. Thus, although the increase in sodium channel expression induced by NGF and basic FGF parallels the changes in morphology that lead to neurite outgrowth, it clearly does not depend on them. The results show that different aspects of neuronal differentiation might be independently regulated by the microenvironment.

This article has been cited by other articles:

				C. P. Ford, K. V. Wong, V. B. Lu, E. Posse de Chaves, and P. A. Smith Differential Neurotrophic Regulation of Sodium and Calcium Channels in an Adult Sympathetic Neuron J Neurophysiol, March 1, 2008; 99(3): 1319 - 1332. [Abstract] [Full Text] [PDF]

				B. Plouffe, M.-O. Guimond, H. Beaudry, and N. Gallo-Payet Role of Tyrosine Kinase Receptors in Angiotensin II AT2 Receptor Signaling: Involvement in Neurite Outgrowth and in p42/p44mapk Activation in NG108-15 Cells Endocrinology, October 1, 2006; 147(10): 4646 - 4654. [Abstract] [Full Text] [PDF]

				J. A. Neubauer and J. Sunderram Oxygen-sensing neurons in the central nervous system J Appl Physiol, January 1, 2004; 96(1): 367 - 374. [Abstract] [Full Text] [PDF]

				L. W. Kutcher, S. R. Beauman, E. I. Gruenstein, M. A. Kaetzel, and J. R. Dedman Nuclear CaMKII inhibits neuronal differentiation of PC12 cells without affecting MAPK or CREB activation Am J Physiol Cell Physiol, June 1, 2003; 284(6): C1334 - C1345. [Abstract] [Full Text] [PDF]

				X.-P. Chu, J. Miesch, M. Johnson, L. Root, X.-M. Zhu, D. Chen, R. P. Simon, and Z.-G. Xiong Proton-Gated Channels in PC12 Cells J Neurophysiol, May 1, 2002; 87(5): 2555 - 2561. [Abstract] [Full Text] [PDF]

				M. J. Davis, X. Wu, T. R. Nurkiewicz, J. Kawasaki, P. Gui, M. A. Hill, and E. Wilson Regulation of ion channels by protein tyrosine phosphorylation Am J Physiol Heart Circ Physiol, November 1, 2001; 281(5): H1835 - H1862. [Abstract] [Full Text] [PDF]

				O. Shmueli, A. Gdalyahu, K. Sorokina, E. Nevo, A. Avivi, and O. Reiner DCX in PC12 cells: CREB-mediated transcription and neurite outgrowth Hum. Mol. Genet., May 1, 2001; 10(10): 1061 - 1070. [Abstract] [Full Text] [PDF]

				K. TAKAHASHI and Y. OKAMURA Ion Channels and Early Development of Neural Cells Physiol Rev, April 1, 1998; 78(2): 307 - 337. [Abstract] [Full Text] [PDF]

				C. Meisinger, C. Zeschnigk, and C. Grothe In Vivo and in Vitro Effect of Glucocorticoids on Fibroblast Growth Factor (FGF)-2 and FGF Receptor 1Expression J. Biol. Chem., July 12, 1996; 271(28): 16520 - 16525. [Abstract] [Full Text] [PDF]

				J. D. Pollock and S. G. Rane p21[IMAGE] Signaling Is Necessary but Not Sufficient to Mediate Neurotrophin Induction of Calcium Channels in PC12 Cells J. Biol. Chem., April 5, 1996; 271(14): 8008 - 8014. [Abstract] [Full Text] [PDF]

				H. Yao, K. Labudda, C. Rim, P. Capodieci, M. Loda, and P. J. S. Stork Cyclic Adenosine Monophosphate Can Convert Epidermal Growth Factor into a Differentiating Factor in Neuronal Cells J. Biol. Chem., September 1, 1995; 270(35): 20748 - 20753. [Abstract] [Full Text] [PDF]

				H. Xu and M. Goldfarb Multiple Effector Domains within SNT1 Coordinate ERK Activation and Neuronal Differentiation of PC12 Cells J. Biol. Chem., April 13, 2001; 276(16): 13049 - 13056. [Abstract] [Full Text] [PDF]

				M. Tsuji, O. Inanami, and M. Kuwabara Induction of Neurite Outgrowth in PC12 Cells by alpha -Phenyl-N-tert-butylnitron through Activation of Protein Kinase C and the Ras-Extracellular Signal-regulated Kinase Pathway J. Biol. Chem., August 24, 2001; 276(35): 32779 - 32785. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help