QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gray, D. B. Articles by Pilar, G. Search for Related Content PubMed PubMed Citation Articles by Gray, D. B. Articles by Pilar, G.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 10, 2687-2698, Copyright © 1990 by Society for Neuroscience

ARTICLE

Endogenous modulation of ACh release by somatostatin and the differential roles of Ca2+ channels

DB Gray, D Zelazny, N Manthay and G Pilar
Department of Physiology and Neurobiology, University of Connecticut, Storrs 06269.

The classical neurotransmitter acetylcholine (ACh) and the potential modulatory peptide somatostatin are colocalized in terminals of avian choroid neurons. We previously showed that exogenous somatostatin inhibits ACh release in the choroid coat (Gray et al., 1989b). In the present work we determine whether endogenous somatostatin plays a role in neuromodulation and what mechanisms are involved. To determine its role and its mode of secretion, voltage-sensitive Ca2+ channels in these terminals were identified pharmacologically using Ca2(+)- dependent K(+)-evoked ACh release. Release of the primary transmitter ACh was triggered in the presence of high K+ by Ca2+ influx primarily via dihydropyridine (DHP)-insensitive channels, while inhibition of ACh release occurred when L-type channels were activated by the DHP agonist Bay K 8644. The somatostatin antagonist cyclo(7-aminoheptanoyl-phe-D- trp-lys-thr (BZL)) (CyCam) blocks the inhibition of ACh release induced by the agonist Bay K 8644 and indicates that endogenous somatostatin may normally modulate ACh release. Additionally, nifedipine, a DHP antagonist, and pertussis toxin, known to antagonize somatostatin's effect on ACh release, both reverse the Bay K 8644 effect on ACh release. Although the release of labeled ACh in the first 3 min collection period was not significantly affected by CyCam or nifedipine alone, release in the first minute was enhanced by 50% in the presence of 10 microM nifedipine. Preincubation with CyCam alone also increased ACh release. These results support the hypothesis that endogenous somatostatin is physiologically released during the initial minute of depolarization in high K+ and that this release is mediated by DHP- sensitive Ca2+ channels.

This article has been cited by other articles:

				A. Meir, S. Ginsburg, A. Butkevich, S. G. Kachalsky, I. Kaiserman, R. Ahdut, S. Demirgoren, and R. Rahamimoff Ion Channels in Presynaptic Nerve Terminals and Control of Transmitter Release Physiol Rev, July 1, 1999; 79(3): 1019 - 1088. [Abstract] [Full Text] [PDF]

				M. G. White, M. A. Crumling, and S. D. Meriney Developmental Changes in Calcium Current Pharmacology and Somatostatin Inhibition in Chick Parasympathetic Neurons J. Neurosci., August 15, 1997; 17(16): 6302 - 6313. [Abstract] [Full Text] [PDF]

				Y. Sugiura and C.-P. Ko Novel Modulatory Effect of L-Type Calcium Channels at Newly Formed Neuromuscular Junctions J. Neurosci., February 1, 1997; 17(3): 1101 - 1111. [Abstract] [Full Text] [PDF]

				M. M. Rathouz, S. Vijayaraghavan, and D. K. Berg Acetylcholine Differentially Affects Intracellular Calcium via Nicotinic and Muscarinic Receptors on the Same Population of Neurons J. Biol. Chem., June 16, 1995; 270(24): 14366 - 14375. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help