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Journal of Neuroscience, Vol 10, 2735-2748, Copyright © 1990 by Society for Neuroscience
Alpha-internexin, a novel neuronal intermediate filament protein, precedes the low molecular weight neurofilament protein (NF-L) in the developing rat brain
MP Kaplan, SS Chin, KH Fliegner and RK Liem
Department of Pathology, Columbia University College of Physicians and Surgeons, New York, New York 10032.
alpha-Internexin is a 66 kDa protein that copurifies with intermediate
filaments (IF) from rat spinal cord and optic nerve. This protein is
axonally transported in rat optic nerve along with the neurofilament
triplet proteins in slow component a. Polymerization in vitro and
distribution in vivo confirm that alpha-internexin is a neuronal IF. We
raised 2 highly specific monoclonal antibodies to alpha-internexin which
were applied to frozen rat brain sections and Western blots of cytoskeletal
extracts. These results indicate that alpha-internexin is primarily an
axonal protein found in most, if not all, neurons of the CNS.
Immunoreactive proteins of similar molecular weight were found in
cytoskeletal extracts of CNS tissue from several additional species,
including mouse and cow. While the distribution of alpha-internexin as
given by immunocytochemical methods is similar to that of low molecular
weight neurofilament protein (NF-L) in the adult, its distribution in the
embryo is far more extensive. At embryonic day 16, when the expression of
NF-L is still limited to a relatively small number of cells and levels of
expression are low, alpha-internexin is already found at much higher levels
and in cells not yet expressing NF-L in detectable quantities. Similar
results are found at embryonic day 12. These data suggest that neuronal IF
in the developing nervous system contain a higher proportion of
alpha-internexin than their adult counterparts, and that expression of
alpha-internexin precedes that of NF-L in many or most neurons of the
developing brain.
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