 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 10, 2903-2916, Copyright © 1990 by Society for Neuroscience
The role of the divergent amino and carboxyl domains on the inactivation properties of potassium channels derived from the Shaker gene of Drosophila
LE Iverson and B Rudy
Division of Neurosciences, Beckman Research Institute of the City of Hope, Duarte, California 91010.
Several products generated from the Drosophila Shaker gene by alternative
splicing predict a group of similar proteins with an identical central and
variable amino and carboxyl domains. We have constructed 9 Sh cDNAs
combining 3 different 5' domains with 3 different 3' domains. RNA
transcribed from 6 of these cDNAs induce K+ currents in Xenopus oocytes.
All currents share similar properties of voltage dependence, potassium
selectivity, and block by 4-AP, TEA, and charybdotoxin. These properties
presumably result from a channel core formed by the identical central
region of the proteins. The currents differ in macroscopic inactivation
kinetics. Five RNAs induced K+ currents which inactivate, each at distinct
rates, during short depolarizations. The sixth RNA induces a current that
essentially does not inactivate unless depolarized for many seconds. This
raises the possibility that Sh may encode nontransient as well as transient
K+ currents. Analysis of currents produced by the various combinations
suggests that the divergent amino domains influence the stability of a
first, nonabsorbing, inactivated state. This results in striking
differences in the probability of channel reopening, as observed in
single-channel recordings, of those channels with identical carboxyl but
different amino domains. Furthermore, based on macroscopic analysis of the
currents, we suggest that the primary role of the carboxyl domains is to
influence the relative stability between the first and a second inactivated
state. The second inactivated state is essentially absorbing, and recovery
from this state is very slow. The observed differences in the rates of
recovery from inactivation of channels containing different carboxyl
domains reflect differences in the rates at which they enter this second
inactivated state.
This article has been cited by other articles:
|
|
|
|
 
G. Gasque, P. Labarca, E. Reynaud, and A. Darszon
Shal and Shaker Differential Contribution to the K+ Currents in the Drosophila Mushroom Body Neurons
J. Neurosci.,
March 2, 2005;
25(9):
2348 - 2358.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. G. Wustenberg, M. Boytcheva, B. Grunewald, J. H. Byrne, R. Menzel, and D. A. Baxter
Current- and Voltage-Clamp Recordings and Computer Simulations of Kenyon Cells in the Honeybee
J Neurophysiol,
October 1, 2004;
92(4):
2589 - 2603.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. H. Jerng and W. F. Gilly
Inactivation and Pharmacological Properties of sqKv1A Homotetramers in Xenopus Oocytes Cannot Account for Behavior of the Squid "Delayed Rectifier" K+ Conductance
Biophys. J.,
June 1, 2002;
82(6):
3022 - 3036.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Z. Varga, M. D. Rayner, and J. G. Starkus
Cations Affect the Rate of Gating Charge Recovery in Wild-type and W434F Shaker Channels through a Variety of Mechanisms
J. Gen. Physiol.,
April 29, 2002;
119(5):
467 - 486.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Lisbin, J. Qiu, and K. White
The neuron-specific RNA-binding protein ELAV regulates neuroglian alternative splicing in neurons and binds directly to its pre-mRNA
Genes & Dev.,
October 1, 2001;
15(19):
2546 - 2561.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Vega-Saenz de Miera, D. H. P. Lau, M. Zhadina, D. Pountney, W. A. Coetzee, and B. Rudy
KT3.2 and KT3.3, Two Novel Human Two-Pore K+ Channels Closely Related to TASK-1
J Neurophysiol,
July 1, 2001;
86(1):
130 - 142.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-D. Yao and C.-F. Wu
Distinct Roles of CaMKII and PKA in Regulation of Firing Patterns and K+ Currents in Drosophila Neurons
J Neurophysiol,
April 1, 2001;
85(4):
1384 - 1394.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Saganich, E. V.-S. de Miera, M. S. Nadal, H. Baker, W. A. Coetzee, and B. Rudy
Cloning of Components of a Novel Subthreshold-Activating K+ Channel with a Unique Pattern of Expression in the Cerebral Cortex
J. Neurosci.,
December 15, 1999;
19(24):
10789 - 10802.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W.-D. Yao and C.-F. Wu
Auxiliary Hyperkinetic beta Subunit of K+ Channels: Regulation of Firing Properties and K+ Currents in Drosophila Neurons
J Neurophysiol,
May 1, 1999;
81(5):
2472 - 2484.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Pelz, J. Jander, H. Rosenboom, M. Hammer, and R. Menzel
IA in Kenyon Cells of the Mushroom Body of Honeybees Resembles Shaker Currents: Kinetics, Modulation by K+, and Simulation
J Neurophysiol,
April 1, 1999;
81(4):
1749 - 1759.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. G. Mermelstein, W.-J. Song, T. Tkatch, Z. Yan, and D. J. Surmeier
Inwardly Rectifying Potassium (IRK) Currents Are Correlated with IRK Subunit Expression in Rat Nucleus Accumbens Medium Spiny Neurons
J. Neurosci.,
September 1, 1998;
18(17):
6650 - 6661.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. N. Uebele, S. K. England, D. J. Gallagher, D. J. Snyders, P. B. Bennett, and M. M. Tamkun
Distinct domains of the voltage-gated K+ channel Kvbeta 1.3 beta -subunit affect voltage-dependent gating
Am J Physiol Cell Physiol,
June 1, 1998;
274(6):
C1485 - C1495.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. J. C. Rosenthal, T. I. Liu, and W. F. Gilly
A Family of Delayed Rectifier Kv1 cDNAs Showing Cell Type-Specific Expression in the Squid Stellate Ganglion/Giant Fiber Lobe Complex
J. Neurosci.,
July 1, 1997;
17(13):
5070 - 5079.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Rogero, B. Hammerle, and F. J. Tejedor
Diverse Expression and Distribution of Shaker Potassium Channels during the Development of the Drosophila Nervous System
J. Neurosci.,
July 1, 1997;
17(13):
5108 - 5118.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C.-C. Kuo
Deactivation Retards Recovery from Inactivation in Shaker K+ Channels
J. Neurosci.,
May 15, 1997;
17(10):
3436 - 3444.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S Zaffran, M Astier, D Gratecos, and M Semeriva
The held out wings (how) Drosophila gene encodes a putative RNA-binding protein involved in the control of muscular and cardiac activity
Development,
January 5, 1997;
124(10):
2087 - 2098.
[Abstract]
[PDF]
|
|
|
|
|
|
|
N. Schoppa, K McCormack, M. Tanouye, and F. Sigworth
The size of gating charge in wild-type and mutant Shaker potassium channels
Science,
March 27, 1992;
255(5052):
1712 - 1715.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Z. Varga, M. D. Rayner, and J. G. Starkus
Cations Affect the Rate of Gating Charge Recovery in Wild-type and W434F Shaker Channels through a Variety of Mechanisms
J. Gen. Physiol.,
April 29, 2002;
119(5):
467 - 486.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
