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Journal of Neuroscience, Vol 11, 3079-3095, Copyright © 1991 by Society for Neuroscience
Voltage-sensitive potassium channels in Drosophila photoreceptors
RC Hardie
Cambridge University, Department of Zoology, United Kingdom.
A preparation of dissociated Drosophila ommatidia is described that allows
single-channel and whole-cell patch-clamp analysis of currents in
identified sensory neurons. Three distinct classes of voltage- sensitive
potassium conductances are characterized; all were detected in distal parts
of ommatidia from sevenless mutants and hence in one cell class (R1-6
photoreceptors). Rapidly inactivating A-channels (IA), coded by the Shaker
gene, were isolated in multichannel patches from adult flies. While showing
similar kinetics to muscle A-channels, they differ from previously
characterized wild-type Shaker channels in having a much more negative
voltage operating range, being half- inactivated at approximately -70 mV.
Two delayed rectifier conductances were characterized in whole-cell
recordings from pupal photoreceptors. The most commonly encountered class
(IKs) is similar to previously reported delayed rectifier conductances in
Drosophila. It inactivates slowly (time constant, approximately 500 msec)
and is half-inactivated at approximately -40 mV. A more rapidly
inactivating delayed rectifier (IKf) was detected in approximately 30% of
cells; it is half- inactivated at approximately -80 mV. Both IKs and IKf
are blocked by 100 microM quinidine. Neither are greatly affected by
4-aminopyridine, which blocks IA at 1-5 mM. None of the three conductances
was calcium dependent, nor were they obviously affected by the eag
mutation, which affects K channels in muscle. The developmental profile of
the channels is the inverse of that described in muscle. Both IKs and IKf
are present at the earliest pupal stages examined (approximately 60 hr),
but IA was only first detected at approximately 76 hr.(ABSTRACT TRUNCATED
AT 250 WORDS)
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