QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Gottmann, K. Articles by Lux, H. D. Search for Related Content PubMed PubMed Citation Articles by Gottmann, K. Articles by Lux, H. D.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 11, 3371-3378, Copyright © 1991 by Society for Neuroscience

ARTICLE

Distribution of Ca2+ and Na+ conductances during neuronal differentiation of chick DRG precursor cells

K Gottmann, H Rohrer and HD Lux
Department of Neurophysiology, Max Planck Institute for Psychiatry, Planegg-Martinsried, Germany.

The distribution of Ca2+ and Na+ conductances on neuronal precursor cells was investigated during differentiation. Ionic conductances on the soma or on the growth cone were isolated by superfusing all other parts of the cells with sucrose. Conductances on the neuritic shaft were detected as additional conductances after removing sucrose from the neuritis shaft. Neuronal precursor cells were isolated from chick dorsal root ganglia by selectively killing differentiated neurons. Cultured precursor cells differentiated into morphological and functional mature neurons. Functionally undifferentiated precursor cells (during the first 10 hr in culture) expressed only low-voltage- activated (LVA) Ca2+ currents. High-voltage-activated (HVA) Ca2+ and Na+ currents appeared delayed after more than 10 hr in culture. Voltage- dependent conductances, if expressed by a cell, were present on all parts of the surface membrane at all stages of differentiation. LVA Ca2+ conductances were well represented on the growth cone as well as on the soma in functionally undifferentiated precursor cells. During differentiation of precursor cells, LVA Ca2+ and HVA Ca2+ as well as Na+ conductances were expressed on the somatic membrane, on the neuritic shaft, and on the growth cone. These results demonstrate the expression of Ca2+ channels on growth cones during differentiation.

This article has been cited by other articles:

				R. H. Pineda, R. A. Heiser, and A. B. Ribera Developmental, Molecular, and Genetic Dissection of INa In Vivo in Embryonic Zebrafish Sensory Neurons J Neurophysiol, June 1, 2005; 93(6): 3582 - 3593. [Abstract] [Full Text] [PDF]

				S. Schmid and E. Guenther Voltage-Activated Calcium Currents in Rat Retinal Ganglion Cells In Situ: Changes during Prenatal and Postnatal Development J. Neurosci., May 1, 1999; 19(9): 3486 - 3494. [Abstract] [Full Text] [PDF]

				Y. Shitaka, N. Matsuki, H. Saito, and H. Katsuki Basic Fibroblast Growth Factor Increases Functional L-Type Ca2+ Channels in Fetal Rat Hippocampal Neurons: Implications for Neurite Morphogenesis In Vitro J. Neurosci., October 15, 1996; 16(20): 6476 - 6489. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help