QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lu, B. Articles by Black, I. B. Search for Related Content PubMed PubMed Citation Articles by Lu, B. Articles by Black, I. B.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 11, 318-326, Copyright © 1991 by Society for Neuroscience

ARTICLE

NGF gene expression in actively growing brain glia

B Lu, M Yokoyama, CF Dreyfus and IB Black
Department of Neurology, Cornell University Medical College, New York, New York 10021.

Previous work suggested that brain NGF acts locally on cells adjacent to sites of synthesis, in addition to any putative actions on distant, projecting perikarya. To define the basis of local action, we used a sensitive nuclease protection assay to identify cells expressing the NGF gene in vivo and in vitro. In addition to neurons, glia from a variety of developing brain areas synthesized NGF mRNA, suggesting that CNS glia exhibit a generalized capacity to express the gene. Expression was associated with active glial growth. Stimulation of growth with serum increased NGF message 2-fold in culture. Moreover, rapidly growing, low-density glial cultures exhibited 8-fold higher levels of NGF mRNA than quiescent, confluent cultures. The optic nerve, which contains all 3 major types of glia, expressed the message in vivo during neonatal development. In contrast, expression was barely detectable in the adult optic nerve. Transection, which induces glial proliferation, elicited de novo appearance of NGF mRNA in the adult nerve. Our observations suggest that active glial growth is associated with expression of the NGF gene and raise the possibility that actively growing glia in the developing or injured brain regulate neuronal growth through the elaboration of NGF.

This article has been cited by other articles:

				E. S. Levine, R. A. Crozier, I. B. Black, and M. R. Plummer Brain-derived neurotrophic factor modulates hippocampal synaptic transmission by increasing N-methyl-D-aspartic acid receptor activity PNAS, August 18, 1998; 95(17): 10235 - 10239. [Abstract] [Full Text] [PDF]

				E. T. Kavalali, K. S. Hwang, and M. R. Plummer cAMP-Dependent Enhancement of Dihydropyridine-Sensitive Calcium Channel Availability in Hippocampal Neurons J. Neurosci., July 15, 1997; 17(14): 5334 - 5348. [Abstract] [Full Text] [PDF]

				M.-Y. Lee, T. Deller, M. Kirsch, M. Frotscher, and H.-D. Hofmann Differential Regulation of Ciliary Neurotrophic Factor (CNTF) and CNTF Receptor alpha  Expression in Astrocytes and Neurons of the Fascia Dentata after Entorhinal Cortex Lesion J. Neurosci., February 1, 1997; 17(3): 1137 - 1146. [Abstract] [Full Text] [PDF]

				W. J. Friedman, S. Thakur, L. Seidman, and A. B. Rabson Regulation of Nerve Growth Factor mRNA by Interleukin-1 in Rat Hippocampal Astrocytes Is Mediated by NFkappa B J. Biol. Chem., December 6, 1996; 271(49): 31115 - 31120. [Abstract] [Full Text] [PDF]

				R. I. Cohen, R. Marmur, W. T. Norton, M. F. Mehler, and J. A. Kessler Nerve Growth Factor and Neurotrophin-3 Differentially Regulate the Proliferation and Survival of Developing Rat Brain Oligodendrocytes J. Neurosci., October 15, 1996; 16(20): 6433 - 6442. [Abstract] [Full Text] [PDF]

				S. P. Pshenichkin and B. C. Wise Okadaic Acid Increases Nerve Growth Factor Secretion, mRNA Stability, and Gene Transcription in Primary Cultures of Cortical Astrocytes J. Biol. Chem., March 17, 1995; 270(11): 5994 - 5999. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help