 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 11, 435-444, Copyright © 1991 by Society for Neuroscience
Voltage-gated currents in identified rat olfactory receptor neurons
PQ Trombley and GL Westbrook
Department of Biology, University of Oregon, Portland.
Whole-cell recording techniques were used to characterize voltage-gated
membrane currents in neonatal rat olfactory receptor neurons (ORNs) in cell
culture. Mature ORNs were identified in culture by their characteristic
bipolar morphology, by retrograde labeling techniques, and by olfactory
marker protein (OMP) immunoreactivity. ORNs did not have spontaneous
activity, but fired action potentials to depolarizing current pulses.
Action potentials were blocked by tetrodotoxin (TTX), which contrasts with
the TTX-resistant action potentials in salamander olfactory receptor cells
(e.g., Firestein and Werblin, 1987). Prolonged, suprathreshold current
pulses evoked only a single action potential; however, repetitive firing up
to 35 Hz could be elicited by a series of brief depolarizing pulses. Under
voltage clamp, the TTX- sensitive sodium current had activation and
inactivation properties similar to other excitable cells. In TTX and 20 mM
barium, sustained inward current were evoked by voltage steps positive to
-30 mV. This current was blocked by Cd (100 microM) and by nifedipine (IC50
= 368 nM) consistent with L-type calcium channels in other neurons. No
T-type calcium current was observed. Voltage steps positive to -20 mV also
evoked an outward current that did not inactivate during 100-msec
depolarizations. Tail current analysis of this current was consistent with
a selective potassium conductance. The outward current was blocked by
external tetraethylammonium but was unaffected by Cd or 4- aminopyridine
(4-AP) or by removal of external calcium. A transient outward current was
not observed. The 3 voltage-dependent conductances in cultured rat ORNs
appear to be sufficient for 2 essential functions: action potential
generation and transmitter release. As a single odorant-activated channel
can trigger an action potential (e.g., Lynch and Barry, 1989), the
repetitive firing seen with brief depolarizing pulses suggests that ORNs do
not integrate sensory input, but rather act as high-fidelity relays such
that each opening of an odorant- activated channel reaches the olfactory
bulb glomeruli as an action potential.
This article has been cited by other articles:
|
|
|
|
 
A. Savigner, P. Duchamp-Viret, X. Grosmaitre, M. Chaput, S. Garcia, M. Ma, and B. Palouzier-Paulignan
Modulation of Spontaneous and Odorant-Evoked Activity of Rat Olfactory Sensory Neurons by Two Anorectic Peptides, Insulin and Leptin
J Neurophysiol,
June 1, 2009;
101(6):
2898 - 2906.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Pinato, J. Rievaj, S. Pifferi, M. Dibattista, L. Masten, and A. Menini
Electroolfactogram Responses from Organotypic Cultures of the Olfactory Epithelium from Postnatal Mice
Chem Senses,
April 1, 2008;
33(4):
397 - 404.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Schrader, S. P. Perrett, L. Ye, and M. J. Friedlander
Substrates for Coincidence Detection and Calcium Signaling for Induction of Synaptic Potentiation in the Neonatal Visual Cortex
J Neurophysiol,
June 1, 2004;
91(6):
2747 - 2764.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. C. Hegg and M. T. Lucero
Dopamine Reduces Odor- and Elevated-K+-Induced Calcium Responses in Mouse Olfactory Receptor Neurons In Situ
J Neurophysiol,
April 1, 2004;
91(4):
1492 - 1499.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Fieni, V. Ghiaroni, R. Tirindelli, P. Pietra, and A. Bigiani
Apical and basal neurones isolated from the mouse vomeronasal organ differ for voltage-dependent currents
J. Physiol.,
October 15, 2003;
552(2):
425 - 436.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. S. Horning and P. Q. Trombley
Zinc and Copper Influence Excitability of Rat Olfactory Bulb Neurons by Multiple Mechanisms
J Neurophysiol,
October 1, 2001;
86(4):
1652 - 1660.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S.-I. Sekizawa, A. S. French, and P. H. Torkkeli
Low-Voltage-Activated Calcium Current Does Not Regulate the Firing Behavior in Paired Mechanosensory Neurons With Different Adaptation Properties
J Neurophysiol,
February 1, 2000;
83(2):
746 - 753.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Kaluza and H Breer
Responsiveness of olfactory neurons to distinct aliphatic aldehydes
J. Exp. Biol.,
January 3, 2000;
203(5):
927 - 933.
[Abstract]
[PDF]
|
|
|
|
|
|
|
G. Vargas and M. T. Lucero
A Method for Maintaining Odor-responsive Adult Rat Olfactory Receptor Neurons in Short-term Culture
Chem Senses,
April 1, 1999;
24(2):
211 - 216.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. C. Puche and M. T. Shipley
Odor-Induced, Activity-Dependent Transneuronal Gene Induction In Vitro: Mediation by NMDA Receptors
J. Neurosci.,
February 15, 1999;
19(4):
1359 - 1370.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Reuter, K. Zierold, W. H. Schroder, and S. Frings
A Depolarizing Chloride Current Contributes to Chemoelectrical Transduction in Olfactory Sensory Neurons In Situ
J. Neurosci.,
September 1, 1998;
18(17):
6623 - 6630.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Q. Trombley
Selective Modulation of GABAA Receptors by Aluminum
J Neurophysiol,
August 1, 1998;
80(2):
755 - 761.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Grill and S. K. Pixley
In Vitro Generation of Adult Rat Olfactory Sensory Neurons and Regulation of Maturation by Coculture with CNS Tissues
J. Neurosci.,
May 1, 1997;
17(9):
3120 - 3127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Kawai, T. Kurahashi, and A. Kaneko
Nonselective Suppression of Voltage-gated Currents by Odorants in the Newt Olfactory Receptor Cells
J. Gen. Physiol.,
February 1, 1997;
109(2):
265 - 272.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. R. Liman and D. P. Corey
Electrophysiological Characterization of Chemosensory Neurons from the Mouse Vomeronasal Organ
J. Neurosci.,
August 1, 1996;
16(15):
4625 - 4637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Kawai and E.-i. Miyachi
Enhancement by T-Type Ca2+ Currents of Odor Sensitivity in Olfactory Receptor Cells
J. Neurosci.,
May 15, 2001;
21(10):
RC144 - RC144.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
