QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Pfaffinger, P. J. Articles by Kandel, E. R. Search for Related Content PubMed PubMed Citation Articles by Pfaffinger, P. J. Articles by Kandel, E. R.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 11, 918-927, Copyright © 1991 by Society for Neuroscience

ARTICLE

Cloning and expression of an Aplysia K+ channel and comparison with native Aplysia K+ currents

PJ Pfaffinger, Y Furukawa, B Zhao, D Dugan and ER Kandel
Howard Hughes Medical Institute, Department of Physiology and Biophysics, Columbia University, College of Physicians and Surgeons, New York, New York 10032.

We describe here the cloning of the Aplysia K+ channel AK01a.AK01a codes for a protein of 515 amino acids, shows considerable homology to other cloned potassium channels, and can be classified as a member of the ShakerK+ channel family. Expression of the AK01a channel in Xenopus oocytes produces a rapidly inactivating outward potassium current (IAK01a) resembling the A-type currents of Drosophila Shaker. Gating for this current is shifted to potentials considerably more positive than the traditional A-currents of Aplysia; we have, however, identified a novel transient potassium current (IAdepoI) in a subset of Aplysia neurons that has similar gating and pharmacological properties to IAK01a.

This article has been cited by other articles:

				S. Buckingham and A. Spencer K(+) currents in cultured neurones from a polyclad flatworm J. Exp. Biol., January 10, 2000; 203(20): 3189 - 3198. [Abstract]

				M. L. Sutherland, S. H. Williams, R. Abedi, P. A. Overbeek, P. J. Pfaffinger, and J. L. Noebels Overexpression of a Shaker-type potassium channel in mammalian central nervous system dysregulates native potassium channel gene expression PNAS, March 2, 1999; 96(5): 2451 - 2455. [Abstract] [Full Text] [PDF]

				M. Kim, D. J. Baro, C. C. Lanning, M. Doshi, J. Farnham, H. S. Moskowitz, J. H. Peck, B. M. Olivera, and R. M. Harris-Warrick Alternative Splicing in the Pore-Forming Region of shaker Potassium Channels J. Neurosci., November 1, 1997; 17(21): 8213 - 8224. [Abstract] [Full Text] [PDF]

				T. Jegla and L. Salkoff A Novel Subunit for Shal K+ Channels Radically Alters Activation and Inactivation J. Neurosci., January 1, 1997; 17(1): 32 - 44. [Abstract] [Full Text] [PDF]

				M. Ma and J. Koester The Role of K+ Currents in Frequency-Dependent Spike Broadening in Aplysia R20 Neurons: A Dynamic-Clamp Analysis J. Neurosci., July 1, 1996; 16(13): 4089 - 4101. [Abstract] [Full Text] [PDF]

				J. Xu, W. Yu, Y. N. Jan, L. Y. Jan, and M. Li Assembly of Voltage-gated Potassium Channels J. Biol. Chem., October 20, 1995; 270(42): 24761 - 24768. [Abstract] [Full Text] [PDF]

				M Li, Y. Jan, and L. Jan Specification of subunit assembly by the hydrophilic amino-terminal domain of the Shaker potassium channel Science, August 28, 1992; 257(5074): 1225 - 1230. [Abstract] [PDF]

				C. Strang, S. J. Cushman, D. DeRubeis, D. Peterson, and P. J. Pfaffinger A Central Role for the T1 Domain in Voltage-gated Potassium Channel Formation and Function J. Biol. Chem., July 20, 2001; 276(30): 28493 - 28502. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help