WWW.JNEUROSCI.ORG
-
The Journal of Neuroscience Synaptic Systems Antibody Company
QUICK SEARCH:   [advanced]


     
-


HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP
This Article
Right arrow Full Text (PDF)
Right arrow Submit an eLetter
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Zufall, F.
Right arrow Articles by Hatt, H.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Zufall, F.
Right arrow Articles by Hatt, H.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 11, 956-965, Copyright © 1991 by Society for Neuroscience

ARTICLE

Ionic currents of cultured olfactory receptor neurons from antennae of male Manduca sexta

F Zufall, M Stengl, C Franke, JG Hildebrand and H Hatt
Physiologisches Institut der Technischen Universitat Munchen, Germany.

Whole-cell and single-channel voltage-clamp techniques were used to identify and characterize the ionic currents of insect olfactory receptor neurons (ORNs) in vitro. The cells were isolated from the antennae of male Manduca sexta pupae at stages 3-5 of adult development and maintained in primary cell culture. After 2-3 weeks in vitro, the presumptive ORNs had resting potentials of -62 +/- 12 mV (n = 18) and expressed at least 1 type of Na+ channel and at least 3 types of K+ channels. Na+ currents, recorded in the whole-cell mode, were reversibly blocked by 0.1 microM tetrodotoxin. The predominant type of K+ channel observed was a voltage-activated K+ channel (gamma = 30 pS) with characteristics similar to those of the delayed rectifier. The activity of the 30-pS K+ channel could be inhibited by the application of nucleotides to the cytoplasmic face of inside-out patches of membrane. The nucleotides had relative potencies as follows: ATP greater than cGMP greater than cAMP, with an inhibition constant for ATP of Ki = 0.18 mM. Raising the intracellular Ca2+ concentration from 0.1 to 5 microM induced the opening of a Ca2(+)-activated K+ channel (gamma = 66 pS at 0 mV) that had a low voltage sensitivity. A third, transient type of K+ channel (gamma = 12-18 pS) could be activated by depolarizing voltage steps from very negative resting potentials. Properties of this channel were similar to those of the "A-channel." These results support the conclusion that M. sexta ORNs differentiate in vitro and provide the basis for studying primary mechanisms of olfactory transduction.


This article has been cited by other articles:


Home page
 Chem SensesHome page
J. Dolzer, S. Krannich, and M. Stengl
Pharmacological Investigation of Protein Kinase C- and cGMP-Dependent Ion Channels in Cultured Olfactory Receptor Neurons of the Hawkmoth Manduca sexta
Chem Senses, July 16, 2008; (2008) bjn043v1.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
C. Flecke, J. Dolzer, S. Krannich, and M. Stengl
Perfusion with cGMP analogue adapts the action potential response of pheromone-sensitive sensilla trichoidea of the hawkmoth Manduca sexta in a daytime-dependent manner
J. Exp. Biol., October 1, 2006; 209(19): 3898 - 3912.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
D. G. Wustenberg, M. Boytcheva, B. Grunewald, J. H. Byrne, R. Menzel, and D. A. Baxter
Current- and Voltage-Clamp Recordings and Computer Simulations of Kenyon Cells in the Honeybee
J Neurophysiol, October 1, 2004; 92(4): 2589 - 2603.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
J. Dolzer, K. Fischer, and M. Stengl
Adaptation in pheromone-sensitive trichoid sensilla of the hawkmoth Manduca sexta
J. Exp. Biol., May 1, 2003; 206(9): 1575 - 1588.
[Abstract] [Full Text] [PDF]

Home page
 Chem SensesHome page
P. Lucas and T. Shimahara
Voltage- and Calcium-activated Currents in Cultured Olfactory Receptor Neurons of Male Mamestra brassicae (Lepidoptera)
Chem Senses, September 1, 2002; 27(7): 599 - 610.
[Abstract] [Full Text] [PDF]

Home page
 J. Neurophysiol.Home page
A. R. Mercer and J. G. Hildebrand
Developmental Changes in the Density of Ionic Currents in Antennal-Lobe Neurons of the Sphinx Moth, Manduca sexta
J Neurophysiol, June 1, 2002; 87(6): 2664 - 2675.
[Abstract] [Full Text] [PDF]

Home page
 J. Exp. Biol.Home page
C Benkenstein, M Schmidt, and M Gewecke
Voltage-activated whole-cell K+ currents in lamina cells of the desert locust schistocerca gregaria
J. Exp. Biol., January 7, 1999; 202(14): 1939 - 1951.
[Abstract] [PDF]

Home page
 J. Neurophysiol.Home page
P. Kloppenburg, B. S. Kirchhof, and A. R. Mercer
Voltage-Activated Currents From Adult Honeybee (Apis mellifera) Antennal Motor Neurons Recorded In Vitro and In Situ
J Neurophysiol, January 1, 1999; 81(1): 39 - 48.
[Abstract] [Full Text] [PDF]


-

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help
-
Copyright 2008 by Society for Neuroscience ONLINE ISSN: 1529-2401
-