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Journal of Neuroscience, Vol 11, 979-984, Copyright © 1991 by Society for Neuroscience
Cell dynamics in the adult mouse olfactory epithelium: a quantitative autoradiographic study
A Mackay-Sim and P Kittel
Division of Science and Technology, Griffith University, Nathan, Queensland, Australia.
The dynamics of cell genesis in the olfactory epithelium of the adult mouse
were investigated using 3H-thymidine autoradiography. Mice were injected
once with 3H-thymidine, and their olfactory epithelia were examined 7, 14,
30, 60, and 90 d later. The number of silver grains over each nucleus was
counted, and the relative distance from the basement membrane was measured
for each labeled nucleus. At 7 and 14 d, the average number of labeled
cells in each section was about 20 per mm. By 30 d, and for the following
60 d, the average number of labeled cells was only about 6 per mm. Thus,
most cells labeled by the injection died 2-4 weeks after injection. When
the labeled cells were compared by nuclear grain density, time after
injection (the "survival period"), and distance of the nucleus from the
basement membrane (the "migration distance"), it was apparent that there
was a small population of "nonmigrating" cells that remained close to the
basement membrane. These cells, at first heavily labeled, divided a second
time about 60 d after the 3H-thymidine injection, indicated by a
significant decrease in nuclear grain density. This nonmigrating, slowly
dividing basal cell is probably the neural stem cell, which gives rise to
another stem cell and an olfactory neuron precursor by an asymmetric
division. When the relative numbers of nonmigrating and migrating cells
were compared, the results indicated that, after the asymmetric division,
there are at least 2 or 3 rapid, symmetric divisions of the precursor
cells, producing many immature receptor cells. Most of these die within 4
weeks of the 3H-thymidine injection.(ABSTRACT TRUNCATED AT 250 WORDS)
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