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Journal of Neuroscience, Vol 11, 1243-1255, Copyright © 1991 by Society for Neuroscience
Primary culture of neonatal rat olfactory neurons
GV Ronnett, LD Hester and SH Snyder
Department of Neurology, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.
We have prepared primary cultures of purified neonatal rat olfactory
neurons. Dissociated olfactory epithelial cells are maintained in modified
Eagle's medium with D-valine, cytosine arabinoside, and NGF. NGF is
required for neuronal survival. Immunohistochemical staining is positive
for the neuronal markers vimentin, olfactory marker protein, and
neuron-specific enolase, but negative for the glial markers, glial
fibrillary acidic protein, and S-100 protein. Physiologic concentrations of
odorants stimulate cAMP accumulation in the cells. Because of their
morphology, biochemical composition, and responsiveness to odorants, these
cells should enhance olfactory investigations.
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R. D. Barber, D. E. Jaworsky, K.-W. Yau, and G. V. Ronnett
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C. Moon, Y.-K. Sung, R. Reddy, and G. V. Ronnett
Odorants induce the phosphorylation of the cAMP response element binding protein in olfactory receptor neurons
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December 7, 1999;
96(25):
14605 - 14610.
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C. Moon, P. Jaberi, A. Otto-Bruc, W. Baehr, K. Palczewski, and G. V. Ronnett
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R. J. Grill and S. K. Pixley
In Vitro Generation of Adult Rat Olfactory Sensory Neurons and Regulation of Maturation by Coculture with CNS Tissues
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T. Ingi, G. Chiang, and G. V. Ronnett
The Regulation of Heme Turnover and Carbon Monoxide Biosynthesis in Cultured Primary Rat Olfactory Receptor Neurons
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