 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 11, 2075-2086, Copyright © 1991 by Society for Neuroscience
Immunohistochemical localization of an inositol 1,4,5-trisphosphate receptor, P400, in neural tissue: studies in developing and adult mouse brain
S Nakanishi, N Maeda and K Mikoshiba
Division of Regulation of Macromolecular Function, Osaka University, Suita, Japan.
The immunohistochemical localization of P400/inositol 1,4,5- trisphosphate
(InsP3) receptor protein was studied in developing and adult mouse brain by
using monoclonal antibodies. The developmental expression pattern of
P400/InsP3 receptor protein differed among different classes of neurons. It
was first detected in the somata of immature Purkinje cells at embryonic
day 17, in the ventrolateral region of the posterior vermis in the
cerebellum. Axonal immunoreactivity within the cerebellar nuclei was first
present at postnatal day 3. Neurons in the retrosplenial cortex, the
anterior olfactory nucleus, and the CA1 region of the hippocampus expressed
immunoreactivity earlier than other regions of the brain. In the adult
brain, not only the Purkinje cell but also many other types of cells in
many areas of the brain expressed P400/InsP3 receptor, though to a lesser
extent. These included the neurons in the striatum, globus pallidus,
nucleus accumbens septi, anterior olfactory nucleus, olfactory tubercle,
precommissural hippocampus, hippocampus, substantia nigra, cerebral cortex,
pons, and certain hypothalamic nuclei. Forebrain cortical regions that
receive afferents from the olfactory bulb, such as the anterior olfactory
nucleus, olfactory tubercle, prepiriform cortex, entorhinal cortex, and
amygdala, exhibited distinct immunoreactivity, while olfactory bulb was
almost devoid of staining. Immunoreactivity in the axonal pathways was also
found in the limbic- hypothalamic pathways, strionigral projection, and
part of the corpus callosum. Results of Western blot analysis and 3H-InsP3
binding assay were consistent with the qualitative regional differences of
immunoreactivity demonstrated by immunohistochemical study. The location of
InsP3 receptor in the brain correlates well with the InsP3 binding sites
demonstrated by an autoradiographic study.
This article has been cited by other articles:
|
|
|
|
 
K. Hara, A. Shiga, H. Nozaki, J. Mitsui, Y. Takahashi, H. Ishiguro, H. Yomono, H. Kurisaki, J. Goto, T. Ikeuchi, et al.
Total deletion and a missense mutation of ITPR1 in Japanese SCA15 families
Neurology,
August 19, 2008;
71(8):
547 - 551.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. R. J. Gordon and J. S. Bains
Noradrenaline Triggers Multivesicular Release at Glutamatergic Synapses in the Hypothalamus
J. Neurosci.,
December 7, 2005;
25(49):
11385 - 11395.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Laporte, A. Hui, and I. Laher
Pharmacological Modulation of Sarcoplasmic Reticulum Function in Smooth Muscle
Pharmacol. Rev.,
December 1, 2004;
56(4):
439 - 513.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Y. Choi, C. M. Beaman-Hall, and M. L. Vallano
Granule neurons in cerebellum express distinct splice variants of the inositol trisphosphate receptor that are modulated by calcium
Am J Physiol Cell Physiol,
October 1, 2004;
287(4):
C971 - C980.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Nishihara, H. Yoshida-Komiya, C.-S. Chan, L. Liao, R. L. Davis, B. W. O'Malley, and J. Xu
SRC-1 Null Mice Exhibit Moderate Motor Dysfunction and Delayed Development of Cerebellar Purkinje Cells
J. Neurosci.,
January 1, 2003;
23(1):
213 - 222.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Zirlinger, G. Kreiman, and D. J. Anderson
Amygdala-enriched genes identified by microarray technology are restricted to specific amygdaloid subnuclei
PNAS,
April 24, 2001;
98(9):
5270 - 5275.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y. Konishi, T. Aoki, N. Ohkawa, T. Matsu-ura, K. Mikoshiba, and T.-a. Tamura
Identification of the C-terminal activation domain of the NeuroD-related factor (NDRF)
Nucleic Acids Res.,
June 15, 2000;
28(12):
2406 - 2412.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Narasimhan, I. N. Pessah, and D. J. Linden
Inositol-1,4,5-Trisphosphate Receptor-Mediated Ca Mobilization Is Not Required for Cerebellar Long-Term Depression in Reduced Preparations
J Neurophysiol,
December 1, 1998;
80(6):
2963 - 2974.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Dent, G Raisman, and F. Lai
Expression of type 1 inositol 1,4,5-trisphosphate receptor during axogenesis and synaptic contact in the central and peripheral nervous system of developing rat
Development,
January 3, 1996;
122(3):
1029 - 1039.
[Abstract]
[PDF]
|
|
|
|
|
