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Journal of Neuroscience, Vol 11, 2135-2150, Copyright © 1991 by Society for Neuroscience
Expression of ion channels and mutational effects in giant Drosophila neurons differentiated from cell division-arrested embryonic neuroblasts
M Saito and CF Wu
Department of Biology, University of Iowa, Iowa City 52242.
A culture system of "giant" Drosophila neurons derived from cytokinesis-
arrested embryonic neuroblasts was developed to overcome the technical
difficulties usually encountered in studying small Drosophila neurons.
Cytochalasin B-treated neuroblasts differentiated into giant multinucleated
cells that displayed neuronal morphology and neuron- specific markers (Wu
et al., 1990). Here, we report that these giant neurons express different
excitability patterns and membrane channels similar to those reported in
excitable tissues of Drosophila. Individual neurons exhibited distinct
all-or-none or graded voltage responses upon current injection. Both
current- and voltage-clamp recordings could be performed on the same neuron
because of the large cell size, thus making it possible to elucidate the
functional role of the individual types of channels. By using
pharmacological agents and ion substitution, the following currents were
identified in these giant neurons: inward Na+ and Ca2+ currents and outward
voltage-activated (the A-type and delayed rectifier) and Ca(2+)-activated
K+ currents. In addition, we found a tetrodotoxin (TTX)-sensitive,
Na(+)-dependent outward K+ current and a persistent component of an inward
Na+ current, which have not been reported in Drosophila previously. This
culture system can be used to analyze the mutational perturbations in ion
channels and the resultant alterations in membrane excitability. Neurons
from the mutant slowpoke (slo), which is known to lack a component of the
Ca(2+)-activated K+ currents in muscles, exhibited prolonged action
potentials associated with defects in the Ca(2+)- activated K+ current.
This abnormality appeared to be more severe in the neurites than in the
soma.
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