 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 11, 2303-2313, Copyright © 1991 by Society for Neuroscience
Cloning and characterization of Ca(2+)-dependent and Ca(2+)-independent PKCs expressed in Aplysia sensory cells
KE Kruger, WS Sossin, TC Sacktor, PJ Bergold, S Beushausen and JH Schwartz
Howard Hughes Medical Institute, Columbia University, New York, New York 10032.
We isolated cDNA clones from an Aplysia sensory-cell library encoding two
isoforms of protein kinase C (PKC). Several isozyme-specific regions are
conserved in the Aplysia kinases, notably the variable regions V5 in the
Ca(2+)-dependent PKC (Apl I) and V1 in the Ca(2+)- independent PKC (Apl
II). Neuronal proteins with the properties expected of these two isoforms
can be identified with antibodies raised against peptides synthesized from
the amino acid sequences deduced from the clones. Sacktor and Schwartz
(1990) measured the proportion of kinase activity that can be translocated
to membrane in Aplysia sensory neurons and ganglia by stimuli that produce
the presynaptic facilitation underlying behavioral sensitization. Much less
Apl I and Apl II are translocated, suggesting that still other isoforms of
PKC exist in these cells.
This article has been cited by other articles:
|
|
|
|
 
C. A. Farah, I. Nagakura, D. Weatherill, X. Fan, and W. S. Sossin
Physiological Role for Phosphatidic Acid in the Translocation of the Novel Protein Kinase C Apl II in Aplysia Neurons
Mol. Cell. Biol.,
August 1, 2008;
28(15):
4719 - 4733.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-Y. Hu, Y. Chen, and S. Schacher
Protein Kinase C Regulates Local Synthesis and Secretion of a Neuropeptide Required for Activity-Dependent Long-Term Synaptic Plasticity
J. Neurosci.,
August 15, 2007;
27(33):
8927 - 8939.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W. S. Sossin
Isoform specificity of protein kinase Cs in synaptic plasticity
Learn. Mem.,
April 2, 2007;
14(4):
236 - 246.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
O. Khabour, J. Levenson, L. C. Lyons, L. S. Kategaya, J. Chin, J. H. Byrne, and A. Eskin
Coregulation of Glutamate Uptake and Long-Term Sensitization in Aplysia
J. Neurosci.,
October 6, 2004;
24(40):
8829 - 8837.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Barbas, L. DesGroseillers, V. F. Castellucci, T. J. Carew, and S. Marinesco
Multiple Serotonergic Mechanisms Contributing to Sensitization in Aplysia: Evidence of Diverse Serotonin Receptor Subtypes
Learn. Mem.,
September 1, 2003;
10(5):
373 - 386.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nakhost, N. Kabir, P. Forscher, and W. S. Sossin
Protein Kinase C Isoforms Are Translocated to Microtubules in Neurons
J. Biol. Chem.,
October 18, 2002;
277(43):
40633 - 40639.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. S. Magoski, G. F. Wilson, and L. K. Kaczmarek
Protein Kinase Modulation of a Neuronal Cation Channel Requires Protein-Protein Interactions Mediated by an Src homology 3 Domain
J. Neurosci.,
January 1, 2002;
22(1):
1 - 9.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
F. Manseau, X. Fan, T. Hueftlein, W. S. Sossin, and V. F. Castellucci
Ca2+-Independent Protein Kinase C Apl II Mediates the Serotonin-Induced Facilitation at Depressed Aplysia Sensorimotor Synapses
J. Neurosci.,
February 15, 2001;
21(4):
1247 - 1256.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Fox and P. E. Lloyd
Role of cAMP in the Short-Term Modulation of a Neuromuscular System in Aplysia
J Neurophysiol,
March 1, 2000;
83(3):
1567 - 1579.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Nakhost, J. R. Dyer, A. M. Pepio, X. Fan, and W. S. Sossin
Protein Kinase C Phosphorylated at a Conserved Threonine Is Retained in the Cytoplasm
J. Biol. Chem.,
October 8, 1999;
274(41):
28944 - 28949.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Farr, J. Mathews, D.-F. Zhu, and R. T. Ambron
Inflammation Causes a Long-Term Hyperexcitability in the Nociceptive Sensory Neurons of Aplysia
Learn. Mem.,
May 1, 1999;
6(3):
331 - 340.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
F. Manseau, W. S. Sossin, and V. F. Castellucci
Long-Term Changes in Excitability Induced by Protein Kinase C Activation in Aplysia Sensory Neurons
J Neurophysiol,
March 1, 1998;
79(3):
1210 - 1218.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Sugita, D. A. Baxter, and J. H. Byrne
Modulation of a cAMP/Protein Kinase A Cascade by Protein Kinase C in Sensory Neurons of Aplysia
J. Neurosci.,
October 1, 1997;
17(19):
7237 - 7244.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W S Sossin
An autonomous kinase generated during long-term facilitation in Aplysia is related to the Ca(2+)-independent protein kinase C Apl II.
Learn. Mem.,
January 1, 1997;
3(5):
389 - 401.
[Abstract]
[PDF]
|
|
|
|
|
|
|
P. J. Trotter, J. Pedretti, R. Yates, and D. R. Voelker
Phosphatidylserine Decarboxylase 2 of Saccharomyces cerevisiáe
J. Biol. Chem.,
March 17, 1995;
270(11):
6071 - 6080.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
W S Sossin, T C Sacktor, and J H Schwartz
Persistent activation of protein kinase C during the development of long-term facilitation in Aplysia.
Learn. Mem.,
January 1, 1994;
1(3):
189 - 202.
[Abstract]
[PDF]
|
|
|
|
|
|
|
A. M. Pepio and W. S. Sossin
Membrane Translocation of Novel Protein Kinase Cs Is Regulated by Phosphorylation of the C2 Domain
J. Biol. Chem.,
February 2, 2001;
276(6):
3846 - 3855.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
