 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 11, 2433-2442, Copyright © 1991 by Society for Neuroscience
Isolation and functional characterization of Schwann cells derived from adult peripheral nerve
TK Morrissey, N Kleitman and RP Bunge
Miami Project to Cure Paralysis, University of Miami School of Medicine, Florida 33136.
To facilitate the development of autologous transplantation techniques with
which to test the ability of Schwann cell (ScC) implantations to treat
nervous system injury, we have developed a method for procuring large,
essentially pure populations of ScCs from adult peripheral nerve. By
allowing small explants of peripheral nerve trunk to undergo axonal and
myelin breakdown in vitro, rather than dissociating the nerve immediately
after harvest, we are able to (1) rid the explant of nearly all fibroblasts
and (2) capitalize on the intrinsic ScC mitogenic response to peripheral
nerve degeneration. Here, we describe a method that yields up to 98% pure
ScC populations from adult rat sciatic nerve (based on cell soma and
nuclear morphology, S100 staining, and behavior of dissociated cells on
neurites) at cell yields of greater than 2 x 10(4) cells/mg of starting
nerve weight. The purification technique was successfully applied to human
tissue; human phrenic nerve yielded 98% pure ScC populations at cell yields
of 2 x 10(4) cells/mg of initial nerve weight. Similar to neonatally
derived ScCs, adult rat cells can be expanded in coculture with dorsal root
ganglion (DRG) neurons or in isolation in the presence of glial growth
factor and forskolin. Cells expanded indefinitely on DRG neurons, or up to
10 weeks on chemical mitogens, return to quiescence following removal of
the mitogenic stimulus. Expanded adult-derived rat ScCs retain functional
capacity, as evidenced by their ability to myelinate DRG neurites and to
support regeneration of processes from embryonic rat retinal explants.
This article has been cited by other articles:
|
|
|
|
 
B. Barrette, M.-A. Hebert, M. Filali, K. Lafortune, N. Vallieres, G. Gowing, J.-P. Julien, and S. Lacroix
Requirement of Myeloid Cells for Axon Regeneration
J. Neurosci.,
September 17, 2008;
28(38):
9363 - 9376.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. R. Murcia, G. Delhon, M. J. Gonzalez, M. Vilas, J. A. Ramos-Vara, M. De Las Heras, R. W. Nordhausen, and F. A. Uzal
Cluster of cases of malignant schwannoma in cattle
Vet Rec.,
September 13, 2008;
163(11):
331 - 335.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. N. Maldonado, N. L. Alderson, P. V. Monje, P. M. Wood, and H. Hama
FA2H is responsible for the formation of 2-hydroxy galactolipids in peripheral nervous system myelin
J. Lipid Res.,
January 1, 2008;
49(1):
153 - 161.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
I. S. Han, T. B. Seo, K.-H. Kim, J.-H. Yoon, S.-J. Yoon, and U. Namgung
Cdc2-mediated Schwann cell migration during peripheral nerve regeneration
J. Cell Sci.,
January 15, 2007;
120(2):
246 - 255.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Kenoutis, R. C. Efrose, L. Swevers, A. A. Lavdas, M. Gaitanou, R. Matsas, and K. Iatrou
Baculovirus-mediated gene delivery into Mammalian cells does not alter their transcriptional and differentiating potential but is accompanied by early viral gene expression.
J. Virol.,
April 1, 2006;
80(8):
4135 - 4146.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Liu, C.-H. Chau, H. Liu, B. R. Jang, X. Li, Y.-S. Chan, and D. K. Y. Shum
Upregulation of chondroitin 6-sulphotransferase-1 facilitates Schwann cell migration during axonal growth.
J. Cell Sci.,
March 1, 2006;
119(Pt 5):
933 - 942.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Fouad, L. Schnell, M. B. Bunge, M. E. Schwab, T. Liebscher, and D. D. Pearse
Combining Schwann Cell Bridges and Olfactory-Ensheathing Glia Grafts with Chondroitinase Promotes Locomotor Recovery after Complete Transection of the Spinal Cord
J. Neurosci.,
February 2, 2005;
25(5):
1169 - 1178.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Gautreau, J. Manent, B. Fievet, D. Louvard, M. Giovannini, and M. Arpin
Mutant Products of the NF2 Tumor Suppressor Gene Are Degraded by the Ubiquitin-Proteasome Pathway
J. Biol. Chem.,
August 23, 2002;
277(35):
31279 - 31282.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Takami, M. Oudega, M. L. Bates, P. M. Wood, N. Kleitman, and M. B. Bunge
Schwann Cell But Not Olfactory Ensheathing Glia Transplants Improve Hindlimb Locomotor Performance in the Moderately Contused Adult Rat Thoracic Spinal Cord
J. Neurosci.,
August 1, 2002;
22(15):
6670 - 6681.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. W. Plant, P. F. Currier, E. P. Cuervo, M. L. Bates, Y. Pressman, M. B. Bunge, and P. M. Wood
Purified Adult Ensheathing Glia Fail to Myelinate Axons under Culture Conditions that Enable Schwann Cells to Form Myelin
J. Neurosci.,
July 15, 2002;
22(14):
6083 - 6091.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. J. Chandross, R. I. Cohen, P. Paras Jr, M. Gravel, P. E. Braun, and L. D. Hudson
Identification and Characterization of Early Glial Progenitors Using a Transgenic Selection Strategy
J. Neurosci.,
January 15, 1999;
19(2):
759 - 774.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Hadlock, J. Elisseeff, R. Langer, J. Vacanti, and M. Cheney
A Tissue-Engineered Conduit for Peripheral Nerve Repair
Arch Otolaryngol Head Neck Surg,
October 1, 1998;
124(10):
1081 - 1086.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. Ramon-Cueto, G. W. Plant, J. Avila, and M. B. Bunge
Long-Distance Axonal Regeneration in the Transected Adult Rat Spinal Cord Is Promoted by Olfactory Ensheathing Glia Transplants
J. Neurosci.,
May 15, 1998;
18(10):
3803 - 3815.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Erickson, K. O'Shea, N Ghaboosi, L Loverro, G Frantz, M Bauer, L. Lu, and M. Moore
ErbB3 is required for normal cerebellar and cardiac development: a comparison with ErbB2-and heregulin-deficient mice
Development,
January 12, 1997;
124(24):
4999 - 5011.
[Abstract]
[PDF]
|
|
|
|
