 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 11, 2588-2597, Copyright © 1991 by Society for Neuroscience
Existence of different subtypes of nicotinic acetylcholine receptors in the rat habenulo-interpeduncular system
C Mulle, C Vidal, P Benoit and JP Changeux
UA CNRS D1284 "Neurobiologie Moleculaire," Departement des Biotechnologies, Institut Pasteur, Paris.
Neuronal nicotinic ACh receptors (nAChRs) are present in the rat medial
habenula (MHB) and interpeduncular nucleus (IPN), two brain regions
connected through the fasciculus retroflexus (FR). The goal of the present
study was to compare the electrophysiological and pharmacological
characteristics of nAChRs located at pre- and postsynaptic sites within the
MHB-IPN system. nAChRs located on the soma of IPN neurons were studied
using patch-clamp techniques and a preparation of acutely isolated neurons.
Whole-cell currents evoked by Ach and nicotine showed an intense
rectification at positive membrane potentials. nAChR channels were
relatively nonselective for cations, had a unitary conductance of 35 pS,
and were activated by several nicotinic agonists with the following rank
order: cytisine greater than ACh greater than nicotine greater than
dimethylphenylpiperazinium (DMPP). They were blocked by mecamylamine,
hexamethonium, curare, and dihydro-beta-erythroidine (DHBE), but were
insensitive to alpha- bungarotoxin and neuronal bungarotoxin. In contrast,
nAChRs recorded on the soma of MHB neurons under equivalent experimental
conditions exhibited different characteristics for single-channel
conductance and agonist and antagonist sensitivity. The pharmacological
properties of presynaptic nAChRs in the IPN were analyzed in a rat brain
slice preparation. Stimulation of the FR produced a presynaptic afferent
volley recorded in the rostral subnucleus of the IPN. Nicotinic agonists
decreased the amplitude of the afferent volley with different efficacies:
nicotine greater than cytisine greater than ACh greater than DMPP. The
action of nicotine was insensitive to alpha-bungarotoxin and to neuronal
bungarotoxin, but was blocked by mecamylamine, hexamethonium, curare, and
DHBE, with IC50 values different from those reported for IPN postsynaptic
nAChRs. This study thus demonstrates the functional diversity of nAChRs in
the rat CNS.
This article has been cited by other articles:
|
|
|
|
 
S. R. Grady, M. Moretti, M. Zoli, M. J. Marks, A. Zanardi, L. Pucci, F. Clementi, and C. Gotti
Rodent Habenulo-Interpeduncular Pathway Expresses a Large Variety of Uncommon nAChR Subtypes, But Only the {alpha}3{beta}4 and {alpha}3{beta}3{beta}4 Subtypes Mediate Acetylcholine Release
J. Neurosci.,
February 18, 2009;
29(7):
2272 - 2282.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. X. Albuquerque, E. F. R. Pereira, M. Alkondon, and S. W. Rogers
Mammalian Nicotinic Acetylcholine Receptors: From Structure to Function
Physiol Rev,
January 1, 2009;
89(1):
73 - 120.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
X. Guo and R. A. J. Lester
Regulation of Nicotinic Acetylcholine Receptor Desensitization by Ca2+
J Neurophysiol,
January 1, 2007;
97(1):
93 - 101.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-P. Changeux
The Ferrier Lecture 1998 The molecular biology of consciousness investigated with genetically modified mice
Phil Trans R Soc B,
December 29, 2006;
361(1476):
2239 - 2259.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. B. Ficklin, S. Zhao, and G. Feng
Ubiquilin-1 Regulates Nicotine-induced Up-regulation of Neuronal Nicotinic Acetylcholine Receptors
J. Biol. Chem.,
October 7, 2005;
280(40):
34088 - 34095.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. L. Meyer, Y. Xiao, and K. J. Kellar
Agonist Regulation of Rat alpha 3beta 4 Nicotinic Acetylcholine Receptors Stably Expressed in Human Embryonic Kidney 293 Cells
Mol. Pharmacol.,
September 1, 2001;
60(3):
568 - 576.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. J. Marks, P. Whiteaker, J. Calcaterra, J. A. Stitzel, A. E. Bullock, S. R. Grady, M. R. Picciotto, J.-P. Changeux, and A. C. Collins
Two Pharmacologically Distinct Components of Nicotinic Receptor-Mediated Rubidium Efflux in Mouse Brain Require the beta 2 Subunit
J. Pharmacol. Exp. Ther.,
May 1, 1999;
289(2):
1090 - 1103.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
L. P. Dwoskin, L. Teng, S. T. Buxton, and P. A. Crooks
(S)-(-)-Cotinine, the Major Brain Metabolite of Nicotine, Stimulates Nicotinic Receptors to Evoke [3H]Dopamine Release from Rat Striatal Slices in a Calcium-Dependent Manner
J. Pharmacol. Exp. Ther.,
March 1, 1999;
288(3):
905 - 911.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Xiao, E. L. Meyer, J. M. Thompson, A. Surin, J. Wroblewski, and K. J. Kellar
Rat alpha 3/beta 4 Subtype of Neuronal Nicotinic Acetylcholine Receptor Stably Expressed in a Transfected Cell Line: Pharmacology of Ligand Binding and Function
Mol. Pharmacol.,
August 1, 1998;
54(2):
322 - 333.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Iida, H. Iida, S. Dohi, M. Takenaka, H. Fujiwara, and R. J. Traystman
Mechanisms Underlying Cerebrovascular Effects of Cigarette Smoking in Rats In Vivo • Editorial Comment
Stroke,
August 1, 1998;
29(8):
1656 - 1665.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Zoli, C. Lena, M. R. Picciotto, and J.-P. Changeux
Identification of Four Classes of Brain Nicotinic Receptors Using beta 2 Mutant Mice
J. Neurosci.,
June 15, 1998;
18(12):
4461 - 4472.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. I. Damaj, M. Fei-Yin, M. Dukat, W. Glassco, R. A. Glennon, and B. R. Martin
Antinociceptive Responses to Nicotinic Acetylcholine Receptor Ligands after Systemic and Intrathecal Administration in Mice
J. Pharmacol. Exp. Ther.,
March 1, 1998;
284(3):
1058 - 1065.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
K. A. Stauderman, L. S. Mahaffy, M. Akong, G. Veliçelebi, L. E. Chavez-Noriega, J. H. Crona, E. C. Johnson, K. J. Elliott, A. Gillespie, R. T. Reid, et al.
Characterization of Human Recombinant Neuronal Nicotinic Acetylcholine Receptor Subunit Combinations alpha 2beta 4, alpha 3beta 4 and alpha 4beta 4 Stably Expressed in HEK293 Cells
J. Pharmacol. Exp. Ther.,
February 1, 1998;
284(2):
777 - 789.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
A. B. Harkins and A. P. Fox
Activation of Nicotinic Acetylcholine Receptors Augments Calcium Channel-mediated Exocytosis in Rat Pheochromocytoma (PC12) Cells
J. Gen. Physiol.,
February 1, 1998;
111(2):
257 - 269.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. Teng, P. A. Crooks, S. T. Buxton, and L. P. Dwoskin
Nicotinic-Receptor Mediation of S(-)Nornicotine-Evoked [3H]Overflow from Rat Striatal Slices Preloaded with [3H]Dopamine
J. Pharmacol. Exp. Ther.,
November 1, 1997;
283(2):
778 - 787.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
J. M. Kulak, T. A. Nguyen, B. M. Olivera, and J. M. McIntosh
alpha -Conotoxin MII Blocks Nicotine-Stimulated Dopamine Release in Rat Striatal Synaptosomes
J. Neurosci.,
July 15, 1997;
17(14):
5263 - 5270.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. R. Grady, E. U. Grun, M. J. Marks, and A. C. Collins
Pharmacological Comparison of Transient and Persistent [3H]Dopamine Release from Mouse Striatal Synaptosomes and Response to Chronic L-Nicotine Treatment
J. Pharmacol. Exp. Ther.,
July 1, 1997;
282(1):
32 - 43.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. I. Dávila-García, J. L. Musachio, D. C. Perry, Y. Xiao, A. Horti, E. D. London, R. F. Dannals, and K. J. Kellar
[125I]IPH, an Epibatidine Analog, Binds with High Affinity to Neuronal Nicotinic Cholinergic Receptors
J. Pharmacol. Exp. Ther.,
July 1, 1997;
282(1):
445 - 451.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
R. S. Fitzgerald, M. Shirahata, and T. Ide
Further cholinergic aspects of carotid body chemotransduction of hypoxia in cats
J Appl Physiol,
March 1, 1997;
82(3):
819 - 827.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. Lena and J.-P. Changeux
Role of Ca2+ Ions in Nicotinic Facilitation of GABA Release in Mouse Thalamus
J. Neurosci.,
January 15, 1997;
17(2):
576 - 585.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. E. Chavez-Noriega, J. H. Crona, M. S. Washburn, A. Urrutia, K. J. Elliott, and E. C. Johnson
Pharmacological Characterization of Recombinant Human Neuronal Nicotinic Acetylcholine Receptors halpha 2beta 2, halpha 2beta 4, halpha 3beta 2, halpha 3beta 4, halpha 4beta 2, halpha 4beta 4 and halpha 7 Expressed in Xenopus Oocytes
J. Pharmacol. Exp. Ther.,
January 1, 1997;
280(1):
346 - 356.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
B. Buisson, M. Gopalakrishnan, S. P. Arneric, J. P. Sullivan, and D. Bertrand
Human alpha 4beta 2 Neuronal Nicotinic Acetylcholine Receptor in HEK 293 Cells: A Patch-Clamp Study
J. Neurosci.,
December 15, 1996;
16(24):
7880 - 7891.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J.-P. Changeux, A. Bessis, J.-P. Bourgeois, P.-J. Corringer, A. Devillers-Thiery, J.-L. Eisele, M. Kerszberg, C. Lena, N. Le Novere, M. Picciotto, et al.
Nicotinic Receptors and Brain Plasticity
Cold Spring Harb Symp Quant Biol,
January 1, 1996;
61(0):
343 - 362.
[Abstract]
[PDF]
|
|
|
|
|
|
|
M. Hu, C. B. Bigger, and P. D. Gardner
A Novel Regulatory Element of a Nicotinic Acetylcholine Receptor Gene Interacts with a DNA Binding Activity Enriched in Rat Brain
J. Biol. Chem.,
March 3, 1995;
270(9):
4497 - 4502.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
