QUICK SEARCH:   [advanced] Author: Keyword(s): Year:  Vol:  Page:

HOME  |   SEARCH  |   ARCHIVE  |   SUBSCRIBE  |   CONTACT  |   HELP

This Article Full Text (PDF) Submit an eLetter Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Manis, P. B. Articles by Marx, S. O. Search for Related Content PubMed PubMed Citation Articles by Manis, P. B. Articles by Marx, S. O.

 Previous Article  |  Next Article 

Journal of Neuroscience, Vol 11, 2865-2880, Copyright © 1991 by Society for Neuroscience

ARTICLE

Outward currents in isolated ventral cochlear nucleus neurons

PB Manis and SO Marx
Department of Otolaryngology-Head and Neck Surgery, Johns Hopkins University School of Medicine, Baltimore, Maryland 21205.

Neurons of the ventral cochlear nucleus (VCN) perform diverse information processing tasks on incoming activity from the auditory nerve. We have investigated the cellular basis for functional diversity in VCN cells by characterizing the outward membrane conductances of acutely isolated cells using whole-cell, tight-seal, current- and voltage-clamp techniques. The electrical responses of isolated cells fall into two broad categories. Type 1 cells respond to small depolarizations with a regular train of action potentials. Under voltage clamp, these cells exhibit a noninactivating outward current for voltage steps positive to -35 mV. Analysis of tail currents reveals two exponentially decaying components with slightly different voltage dependence. These currents reverse at -73 mV, near the potassium equilibrium potential of -84 mV, and are blocked by tetraethylammonium (TEA). The major outward current in Type I cells thus appears to be mediated by potassium channels. In contrast to Type I cells, Type II cells respond to small depolarizations with only one to three short- latency action potentials and exhibit strong rectification around -70 mV. Under voltage clamp, these cells exhibit a noninactivating outward current with a threshold near -70 mV. Analysis of tail currents reveals two components with different voltage sensitivity and kinetics. A low- threshold current with slow kinetics is partly activated at rest. This current reverses at -77 mV and is blocked by 4-aminopyridine (4-AP) but is only partly affected by TEA. The other component is a high-threshold current activated by steps positive to -35 mV. This current is blocked by TEA, but not by 4-AP. A simple model based on the voltage dependence and kinetics of the slow low-threshold outward current in Type II cells was developed. The model produces current- and voltage-clamp responses that resemble those recorded experimentally. Our results indicate that the two major classes of acoustic response properties of VCN neurons are in part attributable to the types of outward (potassium) conductances present in these cells. The low-threshold conductance in the Type II (bushy) cells probably plays a role in the preservation of information about the acoustic stimulus phase from the auditory nerve to central auditory nuclei involved in low-frequency sound localization.

This article has been cited by other articles:

				R. A. Eatock, J. Xue, and R. Kalluri Ion channels in mammalian vestibular afferents may set regularity of firing J. Exp. Biol., June 1, 2008; 211(11): 1764 - 1774. [Abstract] [Full Text] [PDF]

				Z. Rusznak, G. Bakondi, K. Pocsai, A. Por, L. Kosztka, B. Pal, D. Nagy, and G. Szucs Voltage-gated Potassium Channel (Kv) Subunits Expressed in the Rat Cochlear Nucleus J. Histochem. Cytochem., May 1, 2008; 56(5): 443 - 465. [Abstract] [Full Text] [PDF]

				Y. Gai and L. H. Carney Influence of Inhibitory Inputs on Rate and Timing of Responses in the Anteroventral Cochlear Nucleus J Neurophysiol, March 1, 2008; 99(3): 1077 - 1095. [Abstract] [Full Text] [PDF]

				M. L. Day, B. Doiron, and J. Rinzel Subthreshold K+ Channel Dynamics Interact With Stimulus Spectrum to Influence Temporal Coding in an Auditory Brain Stem Model J Neurophysiol, February 1, 2008; 99(2): 534 - 544. [Abstract] [Full Text] [PDF]

				S. Yang and A. S. Feng Heterogeneous Biophysical Properties of Frog Dorsal Medullary Nucleus (Cochlear Nucleus) Neurons J Neurophysiol, October 1, 2007; 98(4): 1953 - 1964. [Abstract] [Full Text] [PDF]

				F. V. Chirila, K. C. Rowland, J. M. Thompson, and G. A. Spirou Development of gerbil medial superior olive: integration of temporally delayed excitation and inhibition at physiological temperature J. Physiol., October 1, 2007; 584(1): 167 - 190. [Abstract] [Full Text] [PDF]

				H. M. Brew, J. X. Gittelman, R. S. Silverstein, T. D. Hanks, V. P. Demas, L. C. Robinson, C. A. Robbins, J. McKee-Johnson, S. Y. Chiu, A. Messing, et al. Seizures and Reduced Life Span in Mice Lacking the Potassium Channel Subunit Kv1.2, but Hypoexcitability and Enlarged Kv1 Currents in Auditory Neurons J Neurophysiol, September 1, 2007; 98(3): 1501 - 1525. [Abstract] [Full Text] [PDF]

				X.-J. Cao, S. Shatadal, and D. Oertel Voltage-Sensitive Conductances of Bushy Cells of the Mammalian Ventral Cochlear Nucleus J Neurophysiol, June 1, 2007; 97(6): 3961 - 3975. [Abstract] [Full Text] [PDF]

				B. Yang, R. Desai, and L. K. Kaczmarek Slack and Slick KNa Channels Regulate the Accuracy of Timing of Auditory Neurons J. Neurosci., March 7, 2007; 27(10): 2617 - 2627. [Abstract] [Full Text] [PDF]

				J. X. Gittelman and B. L Tempel Kv1.1-Containing Channels Are Critical for Temporal Precision During Spike Initiation J Neurophysiol, September 1, 2006; 96(3): 1203 - 1214. [Abstract] [Full Text] [PDF]

				A. Klug and L. O. Trussell Activation and Deactivation of Voltage-Dependent K+ Channels During Synaptically Driven Action Potentials in the MNTB J Neurophysiol, September 1, 2006; 96(3): 1547 - 1555. [Abstract] [Full Text] [PDF]

				R. Dodla, G. Svirskis, and J. Rinzel Well-Timed, Brief Inhibition Can Promote Spiking: Postinhibitory Facilitation J Neurophysiol, April 1, 2006; 95(4): 2664 - 2677. [Abstract] [Full Text] [PDF]

				J. Nogueira, M. E. Castello, and A. A. Caputi The role of single spiking spherical neurons in a fast sensory pathway J. Exp. Biol., March 15, 2006; 209(6): 1122 - 1134. [Abstract] [Full Text] [PDF]

				S. Kuwada, D. C. Fitzpatrick, R. Batra, and E.-M. Ostapoff Sensitivity to Interaural Time Differences in the Dorsal Nucleus of the Lateral Lemniscus of the Unanesthetized Rabbit: Comparison With Other Structures J Neurophysiol, March 1, 2006; 95(3): 1309 - 1322. [Abstract] [Full Text] [PDF]

				A. R. A. Rodrigues and D. Oertel Hyperpolarization-Activated Currents Regulate Excitability in Stellate Cells of the Mammalian Ventral Cochlear Nucleus J Neurophysiol, January 1, 2006; 95(1): 76 - 87. [Abstract] [Full Text] [PDF]

				X. Zhang and L. H. Carney Response Properties of an Integrate-and-Fire Model That Receives Subthreshold Inputs Neural Comput., December 1, 2005; 17(12): 2571 - 2601. [Abstract] [Full Text] [PDF]

				M. A. Xu-Friedman and W. G. Regehr Dynamic-Clamp Analysis of the Effects of Convergence on Spike Timing. I. Many Synaptic Inputs J Neurophysiol, October 1, 2005; 94(4): 2512 - 2525. [Abstract] [Full Text] [PDF]

				M. A. Xu-Friedman and W. G. Regehr Dynamic-Clamp Analysis of the Effects of Convergence on Spike Timing. II. Few Synaptic Inputs J Neurophysiol, October 1, 2005; 94(4): 2526 - 2534. [Abstract] [Full Text] [PDF]

				Y. Wang and P. B. Manis Synaptic Transmission at the Cochlear Nucleus Endbulb Synapse During Age-Related Hearing Loss in Mice J Neurophysiol, September 1, 2005; 94(3): 1814 - 1824. [Abstract] [Full Text] [PDF]

				L. L. Scott, P. J. Mathews, and N. L. Golding Posthearing Developmental Refinement of Temporal Processing in Principal Neurons of the Medial Superior Olive J. Neurosci., August 31, 2005; 25(35): 7887 - 7895. [Abstract] [Full Text] [PDF]

				X.-J. Cao and D. Oertel Temperature Affects Voltage-Sensitive Conductances Differentially in Octopus Cells of the Mammalian Cochlear Nucleus J Neurophysiol, July 1, 2005; 94(1): 821 - 832. [Abstract] [Full Text] [PDF]

				G. Svirskis, V. Kotak, D. H. Sanes, and J. Rinzel Sodium Along With Low-Threshold Potassium Currents Enhance Coincidence Detection of Subthreshold Noisy Signals in MSO Neurons J Neurophysiol, June 1, 2004; 91(6): 2465 - 2473. [Abstract] [Full Text] [PDF]

				H. Zhang, S. H. Wu, and J. B. Kelly Regulation of Auditory Responses in the Central Nucleus of the Inferior Colliculus by Tetraethylammonium-Sensitive Potassium Channels J Neurophysiol, May 1, 2004; 91(5): 2194 - 2204. [Abstract] [Full Text] [PDF]

				M. A. Reid, J. Flores-Otero, and R. L. Davis Firing Patterns of Type II Spiral Ganglion Neurons In Vitro J. Neurosci., January 21, 2004; 24(3): 733 - 742. [Abstract] [Full Text] [PDF]

				C. Kopp-Scheinpflug, K. Fuchs, W. R. Lippe, B. L. Tempel, and R. Rubsamen Decreased Temporal Precision of Auditory Signaling in Kcna1-Null Mice: An Electrophysiological Study In Vivo J. Neurosci., October 8, 2003; 23(27): 9199 - 9207. [Abstract] [Full Text] [PDF]

				J. S. Rothman and P. B. Manis Differential Expression of Three Distinct Potassium Currents in the Ventral Cochlear Nucleus J Neurophysiol, June 1, 2003; 89(6): 3070 - 3082. [Abstract] [Full Text] [PDF]

				J. S. Rothman and P. B. Manis Kinetic Analyses of Three Distinct Potassium Conductances in Ventral Cochlear Nucleus Neurons J Neurophysiol, June 1, 2003; 89(6): 3083 - 3096. [Abstract] [Full Text] [PDF]

				J. S. Rothman and P. B. Manis The Roles Potassium Currents Play in Regulating the Electrical Activity of Ventral Cochlear Nucleus Neurons J Neurophysiol, June 1, 2003; 89(6): 3097 - 3113. [Abstract] [Full Text] [PDF]

				C. Kopp-Scheinpflug, S. Dehmel, G. J. Dorrscheidt, and R. Rubsamen Interaction of Excitation and Inhibition in Anteroventral Cochlear Nucleus Neurons That Receive Large Endbulb Synaptic Endings J. Neurosci., December 15, 2002; 22(24): 11004 - 11018. [Abstract] [Full Text] [PDF]

				G. Svirskis, V. Kotak, D. H. Sanes, and J. Rinzel Enhancement of Signal-to-Noise Ratio and Phase Locking for Small Inputs by a Low-Threshold Outward Current in Auditory Neurons J. Neurosci., December 15, 2002; 22(24): 11019 - 11025. [Abstract] [Full Text] [PDF]

				P. D. Dodson, M. C. Barker, and I. D. Forsythe Two Heteromeric Kv1 Potassium Channels Differentially Regulate Action Potential Firing J. Neurosci., August 15, 2002; 22(16): 6953 - 6961. [Abstract] [Full Text] [PDF]

				D. Soares, R. A. Chitwood, R. L. Hyson, and C. E. Carr Intrinsic Neuronal Properties of the Chick Nucleus Angularis J Neurophysiol, July 1, 2002; 88(1): 152 - 162. [Abstract] [Full Text] [PDF]

				M. J. Ferragamo and D. Oertel Octopus Cells of the Mammalian Ventral Cochlear Nucleus Sense the Rate of Depolarization J Neurophysiol, May 1, 2002; 87(5): 2262 - 2270. [Abstract] [Full Text] [PDF]

				R. Bal and D. Oertel Potassium Currents in Octopus Cells of the Mammalian Cochlear Nucleus J Neurophysiol, November 1, 2001; 86(5): 2299 - 2311. [Abstract] [Full Text] [PDF]

				S. M. Gardner, L. O. Trussell, and D. Oertel Correlation of AMPA Receptor Subunit Composition with Synaptic Input in the Mammalian Cochlear Nuclei J. Neurosci., September 15, 2001; 21(18): 7428 - 7437. [Abstract] [Full Text] [PDF]

				S. Sivaramakrishnan and D. L. Oliver Distinct K Currents Result in Physiologically Distinct Cell Types in the Inferior Colliculus of the Rat J. Neurosci., April 15, 2001; 21(8): 2861 - 2877. [Abstract] [Full Text] [PDF]

				R. Kempter, C. Leibold, H. Wagner, and J. L. van Hemmen Formation of temporal-feature maps by axonal propagation of synaptic learning PNAS, March 7, 2001; (2001) 61369698. [Abstract] [Full Text]

				C. Chabbert, J. M. Chambard, A. Sans, and G. Desmadryl Three Types of Depolarization-Activated Potassium Currents in Acutely Isolated Mouse Vestibular Neurons J Neurophysiol, March 1, 2001; 85(3): 1017 - 1026. [Abstract] [Full Text] [PDF]

				S. Parameshwaran, C. E. Carr, and T. M. Perney Expression of the Kv3.1 Potassium Channel in the Avian Auditory Brainstem J. Neurosci., January 15, 2001; 21(2): 485 - 494. [Abstract] [Full Text] [PDF]

				R. Bal and D. Oertel Hyperpolarization-Activated, Mixed-Cation Current (Ih) in Octopus Cells of the Mammalian Cochlear Nucleus J Neurophysiol, August 1, 2000; 84(2): 806 - 817. [Abstract] [Full Text] [PDF]

				Y. Cai, J. McGee, and E. J. Walsh Contributions of Ion Conductances to the Onset Responses of Octopus Cells in the Ventral Cochlear Nucleus: Simulation Results J Neurophysiol, January 1, 2000; 83(1): 301 - 314. [Abstract] [Full Text] [PDF]

				S. H. Wu Physiological Properties of Neurons in the Ventral Nucleus of the Lateral Lemniscus of the Rat: Intrinsic Membrane Properties and Synaptic Responses J Neurophysiol, June 1, 1999; 81(6): 2862 - 2874. [Abstract] [Full Text] [PDF]

				N. L. Golding, M. J. Ferragamo, and D. Oertel Role of Intrinsic Conductances Underlying Responses to Transients in Octopus Cells of the Cochlear Nucleus J. Neurosci., April 15, 1999; 19(8): 2897 - 2905. [Abstract] [Full Text] [PDF]

				S. C. Molitor and P. B. Manis Voltage-Gated Ca2+ Conductances in Acutely Isolated Guinea Pig Dorsal Cochlear Nucleus Neurons J Neurophysiol, March 1, 1999; 81(3): 985 - 998. [Abstract] [Full Text] [PDF]

				M. Rathouz and L. Trussell Characterization of Outward Currents in Neurons of the Avian Nucleus Magnocellularis J Neurophysiol, December 1, 1998; 80(6): 2824 - 2835. [Abstract] [Full Text] [PDF]

				P. Dutar, H. M. Vu, and D. J. Perkel Multiple Cell Types Distinguished by Physiological, Pharmacological, and Anatomic Properties in Nucleus HVc of the Adult Zebra Finch J Neurophysiol, October 1, 1998; 80(4): 1828 - 1838. [Abstract] [Full Text] [PDF]

				D. H. Edwards, S.-R. Yeh, and F. B. Krasne Neuronal coincidence detection by voltage-sensitive electrical synapses PNAS, June 9, 1998; 95(12): 7145 - 7150. [Abstract] [Full Text] [PDF]

				P. H. Smith, P. X. Joris, and T. C. T. Yin Anatomy and Physiology of Principal Cells of the Medial Nucleus of the Trapezoid Body (MNTB) of the Cat J Neurophysiol, June 1, 1998; 79(6): 3127 - 3142. [Abstract] [Full Text] [PDF]

				G. Gamkrelidze, C. Giaume, and K. D. Peusner The Differential Expression of Low-Threshold Sustained Potassium Current Contributes to the Distinct Firing Patterns in Embryonic Central Vestibular Neurons J. Neurosci., February 15, 1998; 18(4): 1449 - 1464. [Abstract] [Full Text] [PDF]

				L. H. Carney and M. Friedman Spatiotemporal Tuning of Low-Frequency Cells in the Anteroventral Cochlear Nucleus J. Neurosci., February 1, 1998; 18(3): 1096 - 1104. [Abstract] [Full Text] [PDF]

				P. X. Joris and T. C. T. Yin Envelope Coding in the Lateral Superior Olive. III. Comparison With Afferent Pathways J Neurophysiol, January 1, 1998; 79(1): 253 - 269. [Abstract] [Full Text] [PDF]

				Z.-L. Mo and R. L. Davis Heterogeneous Voltage Dependence of Inward Rectifier Currents in Spiral Ganglion Neurons J Neurophysiol, December 1, 1997; 78(6): 3019 - 3027. [Abstract] [Full Text] [PDF]

				Y. Cai, E. J. Walsh, and J. McGee Mechanisms of Onset Responses in Octopus Cells of the Cochlear Nucleus: Implications of a Model J Neurophysiol, August 1, 1997; 78(2): 872 - 883. [Abstract] [Full Text] [PDF]

				A. Rees, A. Sarbaz, M. S. Malmierca, and F. E. N. Le Beau Regularity of Firing of Neurons in the Inferior Colliculus J Neurophysiol, June 1, 1997; 77(6): 2945 - 2965. [Abstract] [Full Text] [PDF]

				K. Fujino, K. Koyano, and H. Ohmori Lateral and Medial Olivocochlear Neurons Have Distinct Electrophysiological Properties in the Rat Brain Slice J Neurophysiol, May 1, 1997; 77(5): 2788 - 2804. [Abstract] [Full Text] [PDF]

				R. Kempter, C. Leibold, H. Wagner, and J. L. van Hemmen Formation of temporal-feature maps by axonal propagation of synaptic learning PNAS, March 27, 2001; 98(7): 4166 - 4171. [Abstract] [Full Text] [PDF]

Home  |   Search  |   Archive  |   Subscribe  |   Contact  |   Help