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Journal of Neuroscience, Vol 12, 290-296, Copyright © 1992 by Society for Neuroscience
Modulation by cAMP of a slowly activating potassium channel expressed in Xenopus oocytes
EM Blumenthal and LK Kaczmarek
Interdepartmental Neuroscience Program, Yale University School of Medicine, New Haven, Connecticut 06510.
When expressed in the Xenopus oocyte, the minK protein induces a slowly
activating voltage-dependent potassium current (Isk). We studied the
modulation of this current by altering intracellular cAMP levels and found
that the amplitude of Isk is dramatically increased by treatments that
raise cAMP levels and decreased by agents that lower cAMP levels.
Preinjection of a protein inhibitor of the cAMP-dependent protein kinase
blocked the effects of increased cAMP levels. There were no changes in the
voltage dependence or kinetics of Isk. Mutations that eliminate a potential
phosphorylation site on the minK protein did not block the effects of
activating the kinase. In addition, the membrane capacitance of the oocyte
increased and decreased in parallel with Isk. Our results fit a mechanism
in which channel proteins are selectively inserted into and removed from
the plasma membrane in response to changes in kinase activity.
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