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Journal of Neuroscience, Vol 12, 3862-3868, Copyright © 1992 by Society for Neuroscience
Ectopic expression of ultraviolet-rhodopsins in the blue photoreceptor cells of Drosophila: visual physiology and photochemistry of transgenic animals
R Feiler, R Bjornson, K Kirschfeld, D Mismer, GM Rubin, DP Smith, M Socolich and CS Zuker
Howard Hughes Medical Institute, University of California, San Diego, La Jolla 92093-0649.
We have generated transgenic flies expressing R7 cell-specific opsins in
the major class of photoreceptor cells of the Drosophila retina and
characterized their spectral properties using high-resolution
microspectrophotometry and sensitivity recordings. We show that the Rh3 and
Rh4 opsin genes encode UV-sensitive opsins with similar spectral properties
(lambda max = 345 nm and 375 nm), and that Rh3 corresponds to the R7p and
R7marg class of visual pigments. We have also generated Rh3 and Rh4
isoform-specific antibodies and present an R7 cell map of the Drosophila
retina. In a related set of experiments, we show that it is possible to
coexpress two different visual pigments functionally in the same cell and
produce photoreceptors that display the summed spectral response of the
individual pigments. These findings open up the possibility of tuning an
animal's visual behavior by targeted expression of combinations of opsin
genes to selective types of photoreceptors.
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