 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 12, 3896-3919, Copyright © 1992 by Society for Neuroscience
Olfactory sensory neurons are trophically dependent on the olfactory bulb for their prolonged survival
JE Schwob, KE Szumowski and AA Stasky
Department of Anatomy and Cell Biology, SUNY Health Science Center, Syracuse 13210.
In most neural systems, developing neurons are trophically dependent on
contact with their synaptic target for their survival and for some features
of their differentiation. However, in the olfactory system, it is unclear
whether or not the survival and differentiation of olfactory sensory
neurons depend on contact with the olfactory bulb (normally the sole
synaptic target for these neurons). In order to address this issue, we
examined neuronal life-span and differentiation in adult rats subjected to
unilateral olfactory bulb ablation at least 1 month prior to use. Life-span
of a newly generated cohort of olfactory neurons was determined by labeling
them at their "birth" via the incorporation of 3H-thymidine. In the absence
of the bulb, neurons are continually produced at a twofold greater rate.
However, the epithelium on the ablated side is thinner, indicating that
average neuronal life-span must be reduced in the targetless epithelium.
Indeed, nearly 90% of the labeled neurons disappear from the bulbectomized
side between 5 d and 2 weeks of neuronal age. Moreover, on electron
microscopic examination, olfactory axons are degenerating in large numbers
on the ablated side. Since labeled neurons migrate apically through the
width of the epithelium during this same period, it appears that most, if
not all, neurons on the ablated side have a life-span on the order of 2
weeks or less. In contrast, there is a more moderate degree of neuronal
loss on the unoperated side of the same animals during the first 2 weeks
after tracer injection, and that occurs while the neurons are concentrated
in the deeper half of the epithelium, suggesting that there is a
preexisting population of neurons in the control epithelium that does not
die during this period. Likewise, degenerating axons are much less frequent
on the unoperated side. We conclude that life-span is significantly shorter
for olfactory neurons born in the targetless epithelium and that olfactory
neurons are trophically dependent on the presence of the bulb for their
prolonged survival. Neuronal differentiation in the absence of the bulb was
assessed according to ultrastructural criteria and the pattern of protein
expression using antisera to the growth associated protein GAP-43 and the
olfactory marker protein. By both measures, most neurons in the epithelium
on the bulbectomized side, but not all, are immature.(ABSTRACT TRUNCATED AT
400 WORDS)
This article has been cited by other articles:
|
|
|
|
 
J. Reisert, K.-W. Yau, and F. L. Margolis
Olfactory marker protein modulates the cAMP kinetics of the odour-induced response in cilia of mouse olfactory receptor neurons
J. Physiol.,
December 15, 2007;
585(3):
731 - 740.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. T. Foster and T. J. Bartness
Sympathetic but not sensory denervation stimulates white adipocyte proliferation
Am J Physiol Regulatory Integrative Comp Physiol,
December 1, 2006;
291(6):
R1630 - R1637.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. G. Boyd, R. Doucette, and M. D. Kawaja
Defining the role of olfactory ensheathing cells in facilitating axon remyelination following damage to the spinal cord
FASEB J,
May 1, 2005;
19(7):
694 - 703.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. Shi, C. K. Song, A. Giordano, S. Cinti, and T. J. Bartness
Sensory or sympathetic white adipose tissue denervation differentially affects depot growth and cellularity
Am J Physiol Regulatory Integrative Comp Physiol,
April 1, 2005;
288(4):
R1028 - R1037.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. Moriya-Ito, T. Osada, Y. Ishimatsu, K. Muramoto, T. Kobayashi, and M. Ichikawa
Maturation of Vomeronasal Receptor Neurons In Vitro by Coculture with Accessory Olfactory Bulb Neurons
Chem Senses,
February 1, 2005;
30(2):
111 - 119.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Carter, J. L. MacDonald, and A. J. Roskams
Olfactory Horizontal Basal Cells Demonstrate a Conserved Multipotent Progenitor Phenotype
J. Neurosci.,
June 23, 2004;
24(25):
5670 - 5683.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. S. Wang, J. W. Lewcock, P. Feinstein, P. Mombaerts, and R. R. Reed
Genetic disruptions of O/E2 and O/E3 genes reveal involvement in olfactory receptor neuron projection
Development,
March 15, 2004;
131(6):
1377 - 1388.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. L. Iwema, H. Fang, D. B. Kurtz, S. L. Youngentob, and J. E. Schwob
Odorant Receptor Expression Patterns Are Restored in Lesion-Recovered Rat Olfactory Epithelium
J. Neurosci.,
January 14, 2004;
24(2):
356 - 369.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Goetze, H. Breer, and J. Strotmann
A Long-term Culture System for Olfactory Explants with Intrinsically Fluorescent Cell Populations
Chem Senses,
November 1, 2002;
27(9):
817 - 824.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. E. Schwob, S. Saha, S. L. Youngentob, and B. Jubelt
Intranasal Inoculation with the Olfactory Bulb Line Variant of Mouse Hepatitis Virus Causes Extensive Destruction of the Olfactory Bulb and Accelerated Turnover of Neurons in the Olfactory Epithelium of Mice
Chem Senses,
October 1, 2001;
26(8):
937 - 952.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. L. Youngentob, J. E. Schwob, S. Saha, G. Manglapus, and B. Jubelt
Functional Consequences Following Infection of the Olfactory System by Intranasal Infusion of the Olfactory Bulb Line Varient (OBLV) of Mouse Hepatitis Strain JHM
Chem Senses,
October 1, 2001;
26(8):
953 - 963.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. Larsson and H. Tjalve
Intranasal Instillation of Aflatoxin B1 in Rats: Bioactivation in the Nasal Mucosa and Neuronal Transport to the Olfactory Bulb
Toxicol. Sci.,
June 1, 2000;
55(2):
383 - 391.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. K. McEntire and S. K. Pixley
Olfactory Receptor Neurons in Partially Purified Epithelial Cell Cultures: Comparison of Techniques for Partial Purification and Identification of Insulin as an Important Survival Factor
Chem Senses,
February 1, 2000;
25(1):
93 - 101.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. Kirschenbaum, F. Doetsch, C. Lois, and A. Alvarez-Buylla
Adult Subventricular Zone Neuronal Precursors Continue to Proliferate and Migrate in the Absence of the Olfactory Bulb
J. Neurosci.,
March 15, 1999;
19(6):
2171 - 2180.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Pasterkamp, F. De Winter, A. J. G. D. Holtmaat, and J. Verhaagen
Evidence for a Role of the Chemorepellent Semaphorin III and Its Receptor Neuropilin-1 in the Regeneration of Primary Olfactory Axons
J. Neurosci.,
December 1, 1998;
18(23):
9962 - 9976.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. E. BUCKLAND and A. M. CUNNINGHAM
Alterations in the Neurotrophic Factors BDNF, GDNF and CNTF in the Regenerating Olfactory System
Ann. N.Y. Acad. Sci.,
November 30, 1998;
855(1):
260 - 265.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Sandeman, D. Clarke, D. Sandeman, and M. Manly
Growth-Related and Antennular Amputation-Induced Changes in the Olfactory Centers of Crayfish Brain
J. Neurosci.,
August 15, 1998;
18(16):
6195 - 6206.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. J. G. D. Holtmaat, W. T. J. M. C. Hermens, M. A. F. Sonnemans, R. J. Giger, F. W. Van Leeuwen, M. G. Kaplitt, A. B. Oestreicher, W. H. Gispen, and J. Verhaagen
Adenoviral Vector-Mediated Expression of B-50/GAP-43 Induces Alterations in the Membrane Organization of Olfactory Axon Terminals In Vivo
J. Neurosci.,
September 1, 1997;
17(17):
6575 - 6586.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
E. Weiler and A. I. Farbman
Proliferation in the Rat Olfactory Epithelium: Age-Dependent Changes
J. Neurosci.,
May 15, 1997;
17(10):
3610 - 3622.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. J. Grill and S. K. Pixley
In Vitro Generation of Adult Rat Olfactory Sensory Neurons and Regulation of Maturation by Coculture with CNS Tissues
J. Neurosci.,
May 1, 1997;
17(9):
3120 - 3127.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Quinton, W. Hasan, W. Grant, C. Thrasivoulou, R. E. Quiney, G. M. Besser, and P.-M. G. Bouloux
Gonadotropin-Releasing Hormone Immunoreactivity in the Nasal Epithelia of Adults with Kallmann's Syndrome and Isolated Hypogonadotropic Hypogonadism and in the Early Midtrimester Human Fetus
J. Clin. Endocrinol. Metab.,
January 1, 1997;
82(1):
309 - 314.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B. J. Goldstein and J. E. Schwob
Analysis of the Globose Basal Cell Compartment in Rat Olfactory Epithelium Using GBC-1, a New Monoclonal Antibody against Globose Basal Cells
J. Neurosci.,
June 15, 1996;
16(12):
4005 - 4016.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
