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Journal of Neuroscience, Vol 12, 4066-4079, Copyright © 1992 by Society for Neuroscience
Electrophysiological and morphological properties of rat basolateral amygdaloid neurons in vitro
MS Washburn and HC Moises
Department of Physiology, University of Michigan, Ann Arbor 48109-0622.
Electrophysiological and morphological properties of neurons in the rat
basolateral amygdala (BLA) were assessed using intracellular recordings in
brain slice preparations. The vast majority of cells studied were
identified as pyramidal cells on the basis of their accommodation response
and by a prominent afterhyperpolarization that followed a current-evoked
burst of action potentials. The second class of cells consisted of
late-firing neurons that were distinguished electrophysiologically by their
very negative resting membrane potential (-82 mV) and conspicuous delay in
the onset of spike firing in response to depolarizing current injection.
The third class of cells, termed fast-firing neurons, possessed many of the
features of intrinsic inhibitory interneurons found elsewhere in the brain.
These included very brief action potentials (0.7 msec), a relatively
depolarized resting membrane potential (-62 mV), and spontaneous firing at
a high rate and the absence of spike frequency accommodation. Intracellular
labeling with Lucifer yellow of electrophysiologically identified pyramidal
and late-firing cells showed them to have pyramidal to stellate cells
bodies and spine-covered dendrites. Although having an overall
pyramidal-like morphology, late-firing neurons possessed cells bodies and
dendritic fields that were smaller than those of pyramidal cells. Lucifer
yellow-labeled fast-firing neurons had a nonpyramidal morphology, with
somata that were spherical to multipolar in shape and spine-sparse or
aspiny dendrites. The morphological features of these cells corresponded
closely to those of GABA-containing interneurons that have been described
previously in the rat BLA using immunohistochemical techniques (McDonald,
1985b). Thus, it seems likely that activation of fast-firing neurons
underlies inhibitory synaptic events that are recorded in the rat BLA. Our
data support the conclusion derived from previous anatomical studies that
pyramidal neurons constitute the predominant cell type in the BLA and
function as projection neurons in this region of the amygdala. The
determination of whether late-firing cells constitute a unique class of
projection neurons distinct from pyramidal cells must await the outcome of
studies in which the anatomical terminations of this cell type are
specified.
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