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Journal of Neuroscience, Vol 12, 4634-4641, Copyright © 1992 by Society for Neuroscience
The 25 kDa synaptosomal-associated protein SNAP-25 is the major methionine-rich polypeptide in rapid axonal transport and a major substrate for palmitoylation in adult CNS
DT Hess, TM Slater, MC Wilson and JH Skene
Department of Neurobiology, Stanford University School of Medicine, California 94305.
A conspicuous correlate of the developmental transformation of axonal
growth cones to synaptic terminals is a marked increase in synthesis and
axonal transport of a methionine-rich, acidic polypeptide of approximately
25 kDa. This polypeptide, designated "super protein" (SuP), is the most
prominent species among methionine-labeled proteins conveyed by rapid
axonal transport in mature CNS and PNS neurons of warm- and cold-blooded
vertebrates. We show here that SuP is identical to SNAP-25, a highly
conserved synaptic protein of known primary structure, by
immunoprecipitation with anti-SNAP-25 antiserum of SuP labeled with
35S-methionine and transported by retinal ganglion cells of rat and cat. In
addition, we show that SNAP-25/SuP is the most prominent species among
retinal polypeptides that incorporate 3H- palmitate in vivo, that it is
fatty acylated through a hydroxylamine- labile, thioester bond, and that
palmitoylated SNAP-25/SuP is axonally transported. Thus, SNAP-25/SuP is a
rapidly transported constituent of the presynaptic apparatus and a major
neuronal substrate for long-chain fatty acylation.
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19(9):
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98(2):
794 - 806.
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|
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17(2):
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|
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|
|
|
|
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118(17):
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|
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|
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|
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279(48):
50524 - 50536.
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|
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24(40):
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April 6, 2004;
101(14):
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|
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15(4):
1802 - 1815.
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|
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January 1, 2004;
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|
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M. Husain and B. Moss
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August 15, 2003;
77(16):
9008 - 9019.
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|
|
|
|
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K. Hu, J. Carroll, C. Rickman, and B. Davletov
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|
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|
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|
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|
|
|
|
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|
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|
|
|
|
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|
|
|
|
|
|
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|
|
|
|
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|
|
|
|
|
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April 1, 2000;
80(2):
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[Abstract]
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|
|
|
|
|
|
|
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|
|
|
|
|
|
|
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February 1, 2000;
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[Abstract]
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|
|
|
|
|
|
|
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February 1, 2000;
95(3):
921 - 929.
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|
|
|
|
|
|
|
K. Vogel, J.-P. Cabaniols, and P. A. Roche
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January 28, 2000;
275(4):
2959 - 2965.
[Abstract]
[Full Text]
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|
|
|
|
|
|
|
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J. Cell Sci.,
January 9, 2000;
113(18):
3197 - 3205.
[Abstract]
[PDF]
|
|
|
|
|
|
|
S. Gonzalo, W. K. Greentree, and M. E. Linder
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J. Biol. Chem.,
July 23, 1999;
274(30):
21313 - 21318.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
H. J. Kennedy, A. E. Pouli, E. K. Ainscow, L. S. Jouaville, R. Rizzuto, and G. A. Rutter
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274(19):
13281 - 13291.
[Abstract]
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[PDF]
|
|
|
|
|
|
|
D. K. Koticha, S. J. Huddleston, J. W. Witkin, and G. Baldini
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274(13):
9053 - 9060.
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|
|
|
|
|
|
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S. H. Wong, Y. Xu, T. Zhang, G. Griffiths, S. L. Lowe, V. N. Subramaniam, K. T. Seow, and W. Hong
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January 1, 1999;
10(1):
119 - 134.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
M. Steegmaier, B. Yang, J.-S. Yoo, B. Huang, M. Shen, S. Yu, Y. Luo, and R. H. Scheller
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J. Biol. Chem.,
December 18, 1998;
273(51):
34171 - 34179.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. R. Schulz, J. D. Sasaki, and V. D. Vacquier
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J. Biol. Chem.,
September 18, 1998;
273(38):
24355 - 24359.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. K. Sato, T. Darsow, and S. D. Emr
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Mol. Cell. Biol.,
September 1, 1998;
18(9):
5308 - 5319.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
E. Swanton, J. Sheehan, N. Bishop, S. High, and P. Woodman
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Mol. Biol. Cell,
July 1, 1998;
9(7):
1633 - 1647.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
Y. Morikawa, H. Nishida, K. Misawa, T. Nosaka, A. Miyajima, E. Senba, and T. Kitamura
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July 1, 1998;
92(1):
129 - 135.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
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Seven Novel Mammalian SNARE Proteins Localize to Distinct Membrane Compartments
J. Biol. Chem.,
April 24, 1998;
273(17):
10317 - 10324.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
Y.-X. Wang, N. L. Catlett, and L. S. Weisman
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J. Cell Biol.,
March 9, 1998;
140(5):
1063 - 1074.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
S. Gonzalo and M. E. Linder
SNAP-25 Palmitoylation and Plasma Membrane Targeting Require a Functional Secretory Pathway
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March 1, 1998;
9(3):
585 - 597.
[Abstract]
[Full Text]
|
|
|
|
|
|
|
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J. Biol. Chem.,
February 6, 1998;
273(6):
3422 - 3430.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Fasshauer, H. Otto, W. K. Eliason, R. Jahn, and A. T. Brunger
Structural Changes Are Associated with Soluble N-Ethylmaleimide-sensitive Fusion Protein Attachment Protein Receptor Complex Formation
J. Biol. Chem.,
October 31, 1997;
272(44):
28036 - 28041.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. P. Lemons, D. Chen, A. M. Bernstein, M. K. Bennett, and S.W. Whiteheart
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August 15, 1997;
90(4):
1490 - 1500.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
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J. A. McNew, M. Sogaard, N. M. Lampen, S. Machida, R. R. Ye, L. Lacomis, P. Tempst, J. E. Rothman, and T. H. Sollner
Ykt6p, a Prenylated SNARE Essential for Endoplasmic Reticulum-Golgi Transport
J. Biol. Chem.,
July 11, 1997;
272(28):
17776 - 17783.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Bruns, S. Engers, C. Yang, R. Ossig, A. Jeromin, and R. Jahn
Inhibition of Transmitter Release Correlates with the Proteolytic Activity of Tetanus Toxin and Botulinus Toxin A in Individual Cultured Synapses of Hirudo medicinalis
J. Neurosci.,
March 15, 1997;
17(6):
1898 - 1910.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G. Di Paolo, R. Lutjens, V. Pellier, S. A. Stimpson, M.-H. Beuchat, S. Catsicas, and G. Grenningloh
Targeting of SCG10 to the Area of the Golgi Complex Is Mediated by Its NH2-terminal Region
J. Biol. Chem.,
February 21, 1997;
272(8):
5175 - 5182.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
G Wang, J. Witkin, G Hao, V. Bankaitis, P. Scherer, and G Baldini
Syndet is a novel SNAP-25 related protein expressed in many tissues
J. Cell Sci.,
January 2, 1997;
110(4):
505 - 513.
[Abstract]
[PDF]
|
|
|
|
|
|
|
V. Ravichandran, A. Chawla, and P. A. Roche
Identification of a Novel Syntaxin- and Synaptobrevin/VAMP-binding Protein, SNAP-23, Expressed in Non-neuronal Tissues
J. Biol. Chem.,
June 7, 1996;
271(23):
13300 - 13303.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. C. Williamson, J. L. Halpern, C. Montecucco, J. E. Brown, and E. A. Neale
Clostridial Neurotoxins and Substrate Proteolysis in Intact Neurons
J. Biol. Chem.,
March 29, 1996;
271(13):
7694 - 7699.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Jahn, P.I. Hanson, H. Otto, and G. Ahnert-Hilger
Botulinum and Tetanus Neurotoxins: Emerging Tools for the Study of Membrane Fusion
Cold Spring Harb Symp Quant Biol,
January 1, 1995;
60(0):
329 - 335.
[Abstract]
[PDF]
|
|
|
|
|
|
|
Y. Yang, Z. Xia, and Y. Liu
SNAP-25 Functional Domains in SNARE Core Complex Assembly and Glutamate Release of Cerebellar Granule Cells
J. Biol. Chem.,
September 15, 2000;
275(38):
29482 - 29487.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
K. M. S. Misura, L. C. Gonzalez Jr., A. P. May, R. H. Scheller, and W. I. Weis
Crystal Structure and Biophysical Properties of a Complex between the N-terminal SNARE Region of SNAP25 and Syntaxin 1a
J. Biol. Chem.,
October 26, 2001;
276(44):
41301 - 41309.
[Abstract]
[Full Text]
[PDF]
|
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