 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 12, 4663-4676, Copyright © 1992 by Society for Neuroscience
c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-kit in inhibitory interneurons
K Manova, RF Bachvarova, EJ Huang, S Sanchez, SM Pronovost, E Velazquez, B McGuire and P Besmer
Molecular Biology Department, Sloan Kettering Institute, New York, New York 10021.
The c-kit receptor and its cognate ligand, KL, are encoded at the white
spotting locus (W) and the steel locus (Sl) of the mouse, respectively. Sl
and W mutations affect the same cellular targets in melanogenesis,
gametogenesis and hematopoiesis during embryonic development and in adult
life. c-kit is expressed in cellular targets of W and Sl mutations, whereas
KL is expressed in the microenvironment of these targets. c-kit and KL,
however, are also expressed in tissues and cell types that are not targets
of W and Sl mutations, including the brain. The cerebellum contains a small
number of neural cell types whose developmental origins, pathways of
migration, and synaptic contacts are known. We have investigated the
patterns of expression of the c-kit and KL RNA and protein products in
postnatal cerebellar development of the mouse. In the adult cerebellum,
c-kit RNA and protein expression was evident in basket, stellate, and Golgi
neurons. Most strikingly, the c- kit protein is expressed in the basket
cell axons that form "basket" and "pinceau" structures entwining the
Purkinje cell soma and the initial segment of the Purkinje cell axon. KL
RNA expression was found in Purkinje cells, and the KL protein was detected
in Purkinje cell bodies and dendrites. Soluble KL protein was also present
in c-kit- expressing basket, stellate, and Golgi cells, presumably as a
result of internalization of ligand-receptor complexes. During postnatal
development, c-kit and KL RNA and protein expression in Golgi and Purkinje
neurons, respectively, was evident by day 0 and persisted subsequently.
c-kit expression in basket and stellate cells was detected from their time
of birth, starting at day 4. These results suggest a role for the c-kit
receptor system in postnatal development of the cerebellum.
This article has been cited by other articles:
|
|
|
|
 
Y. N. Tallini, K. S. Greene, M. Craven, A. Spealman, M. Breitbach, J. Smith, P. J. Fisher, M. Steffey, M. Hesse, R. M. Doran, et al.
c-kit expression identifies cardiovascular precursors in the neonatal heart
PNAS,
February 10, 2009;
106(6):
1808 - 1813.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-R. Zhao, H. H. Berra, W.-M. Duan, S. Singhal, J. Mehta, A. V. Apkarian, and J. A. Kessler
Beneficial Effects of Hematopoietic Growth Factor Therapy in Chronic Ischemic Stroke in Rats
Stroke,
October 1, 2007;
38(10):
2804 - 2811.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L.-R. Zhao, S. Singhal, W.-M. Duan, J. Mehta, and J. A. Kessler
Brain Repair by Hematopoietic Growth Factors in a Rat Model of Stroke
Stroke,
September 1, 2007;
38(9):
2584 - 2591.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
N. Kawaguchi, K. Horiuchi, J. D. Becherer, Y. Toyama, P. Besmer, and C. P. Blobel
Different ADAMs have distinct influences on Kit ligand processing: phorbol-ester-stimulated ectodomain shedding of Kitl1 by ADAM17 is reduced by ADAM19
J. Cell Sci.,
March 15, 2007;
120(6):
943 - 952.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
T. Yasuhara, N. Matsukawa, K. Hara, G. Yu, L. Xu, M. Maki, S. U. Kim, and C. V. Borlongan
Transplantation of Human Neural Stem Cells Exerts Neuroprotection in a Rat Model of Parkinson's Disease
J. Neurosci.,
November 29, 2006;
26(48):
12497 - 12511.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. Wouters, K. Smans, and J.-M. Vanderwinden
WZsGreen/+: a new green fluorescent protein knock-in mouse model for the study of KIT-expressing cells in gut and cerebellum
Physiol Genomics,
August 11, 2005;
22(3):
412 - 421.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
M. M. Chi and T. L. Powley
c-Kit mutant mouse behavioral phenotype: altered meal patterns and CCK sensitivity but normal daily food intake and body weight
Am J Physiol Regulatory Integrative Comp Physiol,
November 1, 2003;
285(5):
R1170 - R1183.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
V. C. Broudy
Stem Cell Factor and Hematopoiesis
Blood,
August 15, 1997;
90(4):
1345 - 1364.
[Full Text]
[PDF]
|
|
|
|
|
|
|
T Hirata, E Morii, M Morimoto, T Kasugai, T Tsujimura, S Hirota, Y Kanakura, S Nomura, and Y Kitamura
Stem cell factor induces outgrowth of c-kit-positive neurites and supports the survival of c-kit-positive neurons in dorsal root ganglia of mouse embryos
Development,
January 9, 1993;
119(1):
49 - 56.
[Abstract]
[PDF]
|
|
|
|
|
