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Journal of Neuroscience, Vol 12, 644-651, Copyright © 1992 by Society for Neuroscience
Synaptic plasticity in Drosophila memory and hyperexcitable mutants: role of cAMP cascade
Y Zhong, V Budnik and CF Wu
Department of Biology, University of Iowa, Iowa City 52242.
Activity-dependent synaptic plasticity has been implicated in the
refinement and modification of neural circuits during development and
learning. Previous studies show that activity-induced facilitation and
potentiation are disrupted at larval neuromuscular junctions in the memory
mutants dunce (dnc) and rutabaga (rut) of Drosophila. The diminished
learning-memory capacity and synaptic transmission plasticity have been
associated with altered cAMP levels since dnc affects the cAMP-specific
phosphodiesterase and rut affects adenylate cyclase. In this study, the
morphology of larval motor axon terminals was examined by anti-HRP
immunohistochemistry. It was found that the numbers of terminal
varicosities and branches were increased in dnc mutants, which have
elevated cAMP concentrations. Such increase was suppressed in dnc rut
double mutants by rut mutations, which reduce cAMP synthesis. More profuse
projections of larval motor axons have also been reported in double-mutant
combinations of ether a go-go (eag) and Shaker (Sh) alleles, which display
greatly enhanced nerve activity as a result of reduction in different K+
currents. Therefore, we examined combinations of dnc and rut with eag and
Sh mutations to explore the possible relation between activity- and
cAMP-induced morphological changes. We found that the expanded projections
in dnc were further enhanced in double mutants of dnc with either eag or
Sh, an effect that could again be suppressed by rut. The results provide
evidence for altered plasticity of synaptic morphology in memory mutants
dnc and rut and suggest a role of cAMP cascade in mediating
activity-dependent synaptic plasticity.
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