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Journal of Neuroscience, Vol 12, 1033-1039, Copyright © 1992 by Society for Neuroscience
Cell-specific alternative RNA splicing of an FMRFamide gene transcript in the brain
SE Saunders, E Kellett, K Bright, PR Benjamin and JF Burke
Sussex Neuroscience Research Centre, School of Biological Sciences, University of Sussex, Brighton, United Kingdom.
Individual neurons synthesize different peptide neurotransmitters and
neuromodulators. In general, specificity is achieved by transcriptional
regulation of neuropeptide-encoding genes. In Lymnaea, the FMRFamide and
GDP/SDPFLRFamide neuropeptides are encoded by separate exons. Here we
provide evidence that the two exons are part of the same gene and that in
neurons expressing the gene the two exons are spliced onto a common
upstream exon encoding a hydrophobic leader sequence. In addition, in situ
hybridization data show that there is mutually exclusive cytoplasmic
expression of each of the neuropeptide-encoding exons. Thus, differential
neuropeptide synthesis is likely to be regulated by an alternative splicing
mechanism. The cellular specificity of these splicing events is remarkable
and suggests that cell-specific alternative splicing may be of major
importance in establishing neuronal diversity in this system.
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