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Journal of Neuroscience, Vol 12, 736-749, Copyright © 1992 by Society for Neuroscience
Expression of tenascin in the developing and adult cerebellar cortex
S Bartsch, U Bartsch, U Dorries, A Faissner, A Weller, P Ekblom and M Schachner
Department of Neurobiology, Swiss Federal Institute of Technology, Zurich, Switzerland.
Since tenascin may influence neuronal cell development, we studied its
expression pattern using immunocytochemistry, in situ hybridization,
Northern blot analysis, and immunochemistry in the developing and adult
mouse cerebellar cortex. Tenascin immunoreactivity was detectable in all
layers of the developing cerebellar cortex. In the external granular layer,
only the radially oriented processes of Golgi epithelial cells were
immunoreactive, whereas the densely packed cell bodies were immunonegative.
Tenascin was hardly detectable at contact sites between migrating granule
cells and processes of Golgi epithelial cells. Axons of granule cells in
the molecular layer were immunoreactive, whereas their cell bodies in the
internal granular layer lacked detectable levels of tenascin. By in situ
hybridization, only Golgi epithelial cells and astrocytes of the internal
granular layer and prospective white matter, but not nerve cells, could be
shown to synthesize detectable levels of tenascin mRNA in the developing
mouse cerebellar cortex. Thus, tenascin in the cerebellar cortex seems to
be a glia-derived molecule that becomes adsorbed to neuronal surfaces in a
topographically restricted pattern in situ. Levels of tenascin protein and
mRNA decreased significantly with increasing age. In the adult, tenascin
immunoreactivity was weak and mainly restricted to the molecular layer and
tenascin mRNA was confined to Golgi epithelial cells, indicative for a
functional heterogeneity in differentiated cerebellar astrocytes.
Quantitative immunoblot analysis revealed that the 225 and 240 kDa
components of tenascin were developmentally downregulated at a faster rate
than the 190 and 200 kDa components, corresponding to the faster
downregulation of the 8 kilobase (kb) mRNA species compared to the 6 kb
mRNA species as revealed by Northern blot analysis. These observations
indicate a differentially regulated expression of the tenascin components.
We hypothesize that glia-derived tenascin modifies the functional
properties of nerve cell surfaces and that tenascin is involved in such
different morphogenetic events as neurite growth and oligodendrocyte
distribution.
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