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Journal of Neuroscience, Vol 12, 906-914, Copyright © 1992 by Society for Neuroscience
Pharmacological discrimination of N-type from L-type calcium current and its selective modulation by transmitters
DH Cox and K Dunlap
Physiology Department, Tufts University School of Medicine, Boston, Massachusetts 02111.
GABA and norepinephrine inhibit high voltage-activated calcium current in
chick sensory neurons. Using specific pharmacological tools, we have
dissected this current into two components: the major one is omega-
conotoxin sensitive and dihydropyridine resistant (N-type) while the minor
one is dihydropyridine sensitively and omega-conotoxin resistant (L-type).
The ability to selectively eliminate these two components has allowed us to
determine whether the transmitters target the same or different channel
types. Both GABA and norepinephrine modulate the N- type component as
evidenced by their lack of effect on (1) omega- conotoxin-resistant current
and (2) pure L-type tail current, prolonged by a dihydropyridine calcium
channel agonist. This simple pharmacological profile will allow future
tests of the significance of the two channel types in regulating sensory
neuron functions.
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