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Journal of Neuroscience, Vol 12, 915-923, Copyright © 1992 by Society for Neuroscience
Calcium/calmodulin-activated phosphodiesterase expressed in olfactory receptor neurons
FF Borisy, GV Ronnett, AM Cunningham, D Juilfs, J Beavo and SH Snyder
Department of Neuroscience, Howard Hughes Medical Institute, Johns Hopkins Medical Institutions, Baltimore, Maryland 21205.
We show that calmodulin-dependent phosphodiesterase (CAM-PDE) is
selectively expressed in mature olfactory receptor neurons within the
olfactory mucosa. Immunocytochemical staining reveals neuronal
immunoreactivity that is most pronounced within cilia, dendritic knobs, and
axon bundles. Neither sustentacular cells nor basal cells display
immunoreactivity. The extent of loss of neuronal immunoreactivity following
bulbectomy resembles loss of the neuronal population. High- affinity
CAM-PDE activity in olfactory cilia is fivefold greater than in brain, when
assayed at low micromolar cAMP. This activity is depleted in turbinates
following bulbectomy. Olfactory mucosal PDE activity is composed of a
minimum of two major forms. In the absence of Ca(2+), rolipram-sensitive
PDE comprises 65% of total activity. Following stimulation by Ca2+, CAM-PDE
activity is elevated sixfold to become the predominant form, thereby
increasing total activity 300%, with half-maximal effect at 1 microM Ca2+.
We propose that Ca2+ stimulation of CAM-PDE may be necessary for
termination of olfactory signals.
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