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Journal of Neuroscience, Vol 12, 930-945, Copyright © 1992 by Society for Neuroscience
Spatiotemporal patterns of expression of NGF and the low-affinity NGF receptor in rat embryos suggest functional roles in tissue morphogenesis and myogenesis
EF Wheeler and M Bothwell
Department of Physiology and Biophysics, University of Washington School of Medicine, Seattle 98195.
We show here that NGF and its low-affinity receptor (p75NGFR) are expressed
during rat embryogenesis at sites that are known to have important roles in
tissue morphogenesis and myogenesis. The developing skin of the maxilla,
the mandible, and the limb showed very similar patterns of NGF and p75NGFR
expression. However, NGF and p75NGFR expression in the developing limb
initiated at the limb bud stage and was concentrated at proximal and distal
developmental sites that have been reported to be involved in limb
morphogenesis. Expression at the proximal/distal ends of the limb persisted
throughout limb development, with some of the highest levels of expression
occurring at the limb axillary sites, which were not highly innervated. We
have also found p75NGFR expression at sites of mesenchymal/epithelial
interactions in several developing organs that do not appear to have an
adjacent source of NGF and may therefore be sites that bind and respond to
the other members of the NGF family (brain-derived neurotrophic factor and
neurotrophin-3). These organs include the lung, testes, and kidney, where
expression of p75NGFR occurred during the morphogenesis of specific
epithelial structures and was coexpressed with the cell adhesion molecule
NCAM. In addition, we found that NGF and p75NGFR were expressed during
myogenesis. p75NGFR was observed in myoblast cells expressing MyoD1, a
myoblast differentiation marker, and NGF transcripts in cells just adjacent
to the developing myoblasts. When the myoblasts differentiate into
myotubes, p75NGFR and MyoD1 cease to be expressed and the adjacent cells
concomitantly cease to be make NGF. However, NGF and p75NGFR were not
present in the early muscle precursor cells of the myotome of the somites
but were observed in the dermatome and sclerotome, respectively. These
results suggest that NGF and p75NGFR have functional roles in developmental
processes that affect morphogenesis and cell differentiation.
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