 |
 Previous Article | Next Article 
Journal of Neuroscience, Vol 12, 1688-1697, Copyright © 1992 by Society for Neuroscience
Regional distribution and developmental expression of epidermal growth factor and transforming growth factor-alpha mRNA in mouse brain by a quantitative nuclease protection assay
LM Lazar and M Blum
Fishberg Research Center in Neurobiology, Mount Sinai Medical Center, New York, New York 10029.
A solution-hybridization ribonuclease-protection assay was used to identify
epidermal growth factor (EGF) mRNA in mouse brain and to compare the
regional and developmental levels of EGF gene expression in the CNS with
those of its structural homolog, transforming growth factor-alpha
(TGF-alpha). Adult brain regions examined included brainstem, cerebellum,
cerebral cortex, hippocampus, basal hypothalamus, olfactory bulb, olfactory
tubercle, striatum, and thalamus. While both EGF and TGF-alpha mRNAs were
detected in all regions, TGF-alpha mRNA levels were 15-170 times higher,
ranging from 0.39 (cerebellum and cerebral cortex) to 2.93 (striatum) pg
TGF-alpha mRNA/micrograms total cytoplasmic RNA. In contrast, EGF mRNA
levels ranged from 11 to 36 fg EGF mRNA/micrograms, with the highest
regional concentrations observed in olfactory bulb, basal hypothalamus, and
cerebellum. In our comparison between sexes, no significant male-female
differences in EGF or TGF-alpha mRNA levels were observed for any region of
adult brain. However, in the pituitary gland, consisting of both endocrine
and neural elements, EGF and TGF-alpha mRNA levels were significantly
higher in males (234 and 215 fg/micrograms, respectively) than in females
(172 and 118 fg/micrograms, respectively). An examination of growth factor
gene expression in the developing CNS revealed EGF and TGF-alpha mRNAs
detectable as early as embryonic day 14 (earliest time point studied).
While gene expression for both peptides continued into the postnatal
period, EGF and TGF-alpha mRNA levels were nearly equal to adult
concentrations by postnatal day 10. Taken together, our findings provide
evidence for the synthesis of EGF in brain and suggest a role for both EGF
and TGF-alpha in the development and support of the mammalian CNS.
This article has been cited by other articles:
|
|
|
|
 
A. Ivanov, V. Gerzanich, S. Ivanova, R. DenHaese, O. Tsymbalyuk, and J. M. Simard
Adenylate cyclase 5 and KCa1.1 channel are required for EGFR up-regulation of PCNA in native contractile rat basilar artery smooth muscle
J. Physiol.,
January 1, 2006;
570(1):
73 - 84.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Qiu, Y. Takagi, J. Harada, N. Rodrigues, M. A. Moskowitz, D. T. Scadden, and T. Cheng
Regenerative Response in Ischemic Brain Restricted by p21cip1/waf1
J. Exp. Med.,
April 5, 2004;
199(7):
937 - 945.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
D. Caric, H. Raphael, J. Viti, A. Feathers, D. Wancio, and L. Lillien
EGFRs mediate chemotactic migration in the developing telencephalon
Development,
November 1, 2001;
128(21):
4203 - 4216.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
C. S. Weickert, M. J. Webster, T. M. Hyde, M. M. Herman, S. E. Bachus, G. Bali, D. R. Weinberger, and J. E. Kleinman
Reduced GAP-43 mRNA in Dorsolateral Prefrontal Cortex of Patients with Schizophrenia
Cereb Cortex,
February 1, 2001;
11(2):
136 - 147.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
J. Fallon, S. Reid, R. Kinyamu, I. Opole, R. Opole, J. Baratta, M. Korc, T. L. Endo, A. Duong, G. Nguyen, et al.
In vivo induction of massive proliferation, directed migration, and differentiation of neural cells in the adult mammalian brain
PNAS,
December 19, 2000;
97(26):
14686 - 14691.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
P. M. Hermann, R. E. van Kesteren, W. C. Wildering, S. D. Painter, J. M. Reno, J. S. Smith, S. B. Kumar, W. P. M. Geraerts, L. H. Ericsson, A. B. Smit, et al.
Neurotrophic Actions of a Novel Molluscan Epidermal Growth Factor
J. Neurosci.,
September 1, 2000;
20(17):
6355 - 6364.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
L. A. Opanashuk, R. J. Mark, J. Porter, D. Damm, M. P. Mattson, and K. B. Seroogy
Heparin-Binding Epidermal Growth Factor-Like Growth Factor in Hippocampus: Modulation of Expression by Seizures and Anti-Excitotoxic Action
J. Neurosci.,
January 1, 1999;
19(1):
133 - 146.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
A. G. Rabchevsky, J. M. Weinitz, M. Coulpier, C. Fages, M. Tinel, and M.-P. Junier
A Role for Transforming Growth Factor alpha as an Inducer of Astrogliosis
J. Neurosci.,
December 15, 1998;
18(24):
10541 - 10552.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Marmur, J. A. Kessler, G. Zhu, S. Gokhan, and M. F. Mehler
Differentiation of Oligodendroglial Progenitors Derived from Cortical Multipotent Cells Requires Extrinsic Signals Including Activation of gp130/LIFbeta Receptors
J. Neurosci.,
December 1, 1998;
18(23):
9800 - 9811.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
B Egger, H V Carey, F Procaccino, N-N Chai, E P Sandgren, J Lakshmanan, V S Buslon, S W French, M W Buchler, and V E Eysselein
Reduced susceptibility of mice overexpressing transforming growth factor alpha to dextran sodium sulphate induced colitis
Gut,
July 1, 1998;
43(1):
64 - 70.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
|
|
|
R. Rozental, M. Morales, M. F. Mehler, M. Urban, M. Kremer, R. Dermietzel, J. A. Kessler, and D. C. Spray
Changes in the Properties of Gap Junctions during Neuronal Differentiation of Hippocampal Progenitor Cells
J. Neurosci.,
March 1, 1998;
18(5):
1753 - 1762.
[Abstract]
[Full Text]
[PDF]
|
|
|
|
