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Journal of Neuroscience, Vol 12, 1789-1801, Copyright © 1992 by Society for Neuroscience
Multiple Ca2+ currents elicited by action potential waveforms in acutely isolated adult rat dorsal root ganglion neurons
RS Scroggs and AP Fox
Department of Pharmacological and Physiological Sciences, University of Chicago, Illinois 60637.
Ca2+ entry into different diameter cell bodies of dorsal root ganglion
(DRG) neurons depolarized with action potential (AP) waveform commands was
studied using the whole-cell patch-clamp technique and pharmacological
probes. We have previously shown that Ca2+ current expression in DRG neuron
cell bodies depends on cell diameter. In small diameter DRG neurons, L- and
N-type Ca2+ currents usually accounted for most Ca2+ entry during APs as
determined by blockade with nimodipine and omega-conotoxin GVIA
(omega-CgTx). In medium- diameter DRG neurons, T-type Ca2+ currents
accounted for 29% or 54% of Ca2+ entry in cells held at -60 mV or -80 mV,
respectively, based on blockade by amiloride. T-type Ca2+ currents did not
usually contribute to Ca2+ entry in large diameter DRG neurons. An
amiloride/omega-CgTx/nimodipine-resistant Ca2+ current was prominent in
medium diameter DRG neurons, while L- and N- type Ca2+ currents played a
relatively small role in Ca2+ entry. In all DRG neuron sizes, AP-generated
currents were large in amplitude, resulting in significant Ca2+ entry. APs
with slower rates of repolarization increased Ca2+ entry. In DRG neurons
that expressed T- type Ca2+ currents, the duration of Ca2+ current entry
during APs was prolonged, and this prolongation was reduced by amiloride.
Thus, antagonists selective for different Ca2+ channels produced different
patterns of blockade of AP-generated Ca2+ entry in different diameter DRG
cell bodies. Selective Ca2+ channel modulation by neurotransmitters might
be expected to have similar effects.
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