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Journal of Neuroscience, Vol 12, 2051-2064, Copyright © 1992 by Society for Neuroscience
Alternate neuromuscular target selection following the loss of single muscle fibers in Drosophila
S Cash, A Chiba and H Keshishian
Department of Biology, Yale University, New Haven, Connecticut 06511.
The Drosophila embryonic and larval body wall consists of a simple array of
segmental muscle fibers, innervated in a precise manner by identified
neurons. During development motoneurons were forced to find alternate
targets following the selective deletion of a single muscle fiber, the
pleural internal oblique #5. We used backfills, intracellular dyefills, and
immunocytochemistry in embryos and larvae to characterize the normal
motoneurons to the fiber. Deleting the fiber using either a genetic or
laser lesioning method yielded essentially the same result. In nearly half
the cases examined, an ectopically placed neuromuscular projection was
found on either of two neighboring muscle fibers, with one favored eight
times more than the other. The ectopic projection derived from the nerve
branch that normally supplied the deleted muscle fiber 5. Motoneuronal
endings on undeleted muscle fibers elsewhere in the body wall had normal
morphology. The ectopically placed motor terminals accumulated glutamate in
normally sized synaptic boutons, beneath which transmitter sensitivity was
localized. The number of boutons and branches at the ectopic endings did
not differ significantly from those on intact muscle fiber 5s. Also, the
native motoneurons did not alter their arborization sizes in response to a
supernumerary ectopically placed contact. While the orientation of the
individual ectopically placed branches was variable, the ectopic endings
occupied a predictable site on the surrogate muscle fibers. The results
suggest that Drosophila motoneurons can project to body wall destinations
in the absence of their muscle fiber targets, and that alternate muscle
fibers are selected by their proximity. The muscle fibers will support
apparently stable and functional supernumerary motor endings on ectopic
sites, and these inputs do not significantly influence the behavior of the
native motoneurons. The data suggest that Drosophila motoneurons may behave
autonomously when making synapses, and that competition does not play a
major role in the matching of motoneuron to muscle fiber.
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